| Esterase Substrate (Cat. No.) | Properties in Cells | pKa of Product * |
|---|
| BCECF AM (B1150, B1170, B3051) | - Quite well retained
- Released during cytolysis
- pH-sensitive fluorescence
| 7.0 |
| Calcein AM (C1430, C3099, C3100MP) | - Quite well retained
- Released during cytolysis
- Not as pH-sensitive as BCECF
| 5 |
| Carboxyeosin diacetate, succinimidyl ester (CEDA, SE; C22803; Assays for Cell Enumeration, Cell Proliferation and Cell Cycle—Section 15.4) | - Well retained by reaction with amines
- Useful for DAB photoconversion
- Phosphorescent
| <5 † |
| Carboxy-2',7'-dichlorofluorescein diacetate (carboxy-DCFDA, C369) | - Moderately well retained
- Not as pH-sensitive as CFDA
| 4.8 |
| Carboxyfluorescein diacetate (5(6)-CFDA, C195) | - Moderately well retained
- pH-sensitive fluorescence
| 6.4 |
| Carboxyfluorescein diacetate, acetoxymethyl ester (5-CFDA, AM; C1354) | - Easier to load than CFDA yet yields the same product upon hydrolysis
- pH-sensitive fluorescence
| 6.4 |
| Carboxyfluorescein diacetate, succinimidyl ester (5(6)-CFDA, SE; C1157; Assays for Cell Enumeration, Cell Proliferation and Cell Cycle—Section 15.4) | - Well retained by reaction with amines
- Not completely released during cytolysis
- pH-sensitive fluorescence
| 6.4 † |
| CellTracker Green CMFDA (CMFDA; C2925, C7025) | - Well retained by reaction with thiols
- Not completely released during cytolysis
- pH-sensitive fluorescence
| 6.4 † |
| Chloromethyl SNARF-1, acetate (C6826) | - Well retained by reaction with thiols
- Not completely released during cytolysis
- Long-wavelength, pH-sensitive fluorescence
| 7.5 † |
| Fluorescein diacetate (F1303) | - Poorly retained
- pH-sensitive fluorescence
- Inexpensive
| 6.4 |
| Oregon Green 488 carboxylic acid diacetate (carboxy-F2FDA, O6151) | - Moderately well retained
- Not as pH-sensitive as CFDA
| 4.7 |
| * Approximate pKa values in aqueous solvents. The actual pKa of the indicator will vary somewhat depending upon experimental conditions. † pKa of the unconjugated hydrolysis product; after conjugation to an intracellular amine or thiol, the actual pKa value may be different. |