True to its name, the High-Capacity cDNA synthesis kits produce high quality first-strand cDNA from total RNA. The High-Capacity cDNA Reverse Transcription Kit (formerly the High-Capacity cDNA Archive Kit) is designed for synthesizing cDNA for short- or long-term archiving. The kits yield quantitative reverse transcription products from RNA inputs ranging between 0.02 and 2 μg in a 20 μL reaction; it can be scaled up to 10 μg of input RNA in a 100 μL reaction. Downstream applications include real-time PCR, standard PCR, and labeling for microarray analysis.
High-capacity cDNA synthesis kits: formats
The High-Capacity cDNA reverse transcription kits are available in multiple formats:
Benefits of the High-Capacity RNA-to-cDNA Kit
The High-Capacity RNA-to-cDNA kit consolidates the reagents for reverse transcription reaction into two tubes: 2X RT buffer mix and 20X enzyme mix, which contains reverse transcriptase and RNase inhibitor. These components have been optimized to provide high cDNA yields for real-time gene expression analysis. The two-tube format streamlines the workflow by minimizing the number of pipetting steps and is designed for optimum performance in real-time PCR with TaqMan Fast Advanced Master Mix and PowerUp SYBR Green Master Mix.
Comparison of cDNA synthesis kits for qPCR
For exceptional performance, explore SuperScript IV Reverse Transcriptase—available as a standalone enzyme, first-strand cDNA synthesis kit, master mix for qRT-PCR applications, and one-step RT-PCR system to suit all your research needs.
Reverse transcriptases: Selection tool
Use the selection tool to find the right product for your cDNA synthesis experiments
| High-Capacity cDNA Reverse Transcription kit | High-Capacity RNA-to-cDNA kit | SuperScript IV VILO Master Mix | SuperScript IV VILO Master Mix with ezDNase Enzyme | |
| Application | Value choice for routine applications | cDNA synthesis components in two-tube format | Exceptional master mix for two-step RT-qPCR | Exceptional master mix for two-step RT-qPCR with fast and easy gDNA removal |
| Reverse transcriptase | MultiScribe | MultiScribe | SuperScript IV | SuperScript IV |
| Optimal reaction temperature | 37°C | 37°C | 50°C | 50°C |
| Reaction time | 120 min | 30 to 60 min | 10 min | 10 min |
| Ability to work with degraded or inhibitor-containing RNA | Low | Low | High | High |
| Reduced RNase H activity | No | No | Yes | Yes |
| Primers included | Random primers | Random primers | Random primers and oligo(dT)20 | Random primers and oligo(dT)20 |
| Integrated gDNA removal | No | No | No | Yes* |
| Format | RT reaction components in separate tubes | RT reaction components in two-tube format | All RT reaction components premixed | All RT reaction components premixed |
*Formats supplied with ezDNase enzyme