Product box and its contents: 5 tubes of ElectroMAX DH10B Cells, 1 vial of pUC19 DNA, and 2 bottles of SOC medium

What are DH10B competent cells?

The DH10B strain of E. coli is a popular strain for everyday cloning applications due to its high transformation efficiency, its ability to uptake and maintain large plasmids (>30 kb), lack of methylation-dependent restriction systems (MDRS), and blue-white colony screening. DH10B can intake genomic DNA which differentiates it from the DH5α strain. The full DH10B genome was sequenced and published by Durfee et al. in 2008 [1].

Benefits of DH10B competent cells

  • Genomic mutations make these ideal for transformation of cDNA and genomic DNA libraries
  • Blue-white screening for easy identification of colonies with recombinant DNA
  • Available in chemical or electrocompetent format

Which DH10B competent cells are right for you?

 MAX Efficiency DH10B Competent Cells
MAX Efficiency DH10B T1 Phage Resistant Cells*ElectroMAX DH10B CellsElectroMAX DH10B T1 Phage-Resistant Competent Cells
MegaX DH10B T1R Electrocomp Cells
 
Product box and its contents: 5 tubes of MAX Efficiency DH10B Competent Cells, 1 vial of pUC19 DNA, and 2 bottles of SOC medium
Product box and its contents: 21 tubes of One Shot MAX Efficiency DH10B T1 Phage-Resistant Cells, 1 vial of pUC19 DNA, and 1 bottle of SOC medium
Product box and its contents: 5 tubes of ElectroMAX DH10B Cells, 1 vial of pUC19 DNA, and 2 bottles of SOC medium
Product box and its contents: 5 tubes of ElectroMAX DH10B T1 Phage-Resistant Cells, 1 vial of pUC19 DNA, and 2 bottles of SOC medium
Product box and its contents: 5 tubes of MegaX DH10B T1R Electrocomp Cells, 1 vial of pUC19 DNA, and 3 bottles of recovery media
Best for
  • High efficiency competent cells for broad area of applications
  • Broad area of applications
  • Convenient single-use or high-throughput format
  • High transformation efficiency
  • Library construction and other challenging applications 
  • High transformation efficiency
  • Library construction and other challenging applications
  • Highest efficiency
  • Highly demanding cloning
  • Library construction applications
Transformation efficiency>1 x 109 cfu/µg>1 x 109 cfu/µg>1 x 1010 cfu/µg>1 x 1010 cfu/µg>3 x 1010 cfu/µg
Type of transformationChemicalChemicalElectroporationElectroporationElectroporation
Resistance to T1, T5, and φ80 phage contamination––✓––✓✓
Available formats

* Strain also available in high-throughput FlexPlate format.

References
  1. Durfee R, Nelson R, Baldwin S, et al (2008) The Complete Genome Sequence of Escherichia coli DH10B: Insights into the Biology of a Laboratory Workhorse. J Bacteriol 190(7):2597–2606.