Image-iT FX Kits

All-in-one kits for fluorescence imaging The Image-iT FX Kits provide the dyes and reagents you need for optimal immunofluorescence imaging of fixed cells and tissue sections. Each kit includes: Alexa Fluor secondary antibodyor streptavidin conjugates for superior photostability and brightness ProLong Gold antifade reagent for reduced photobleaching Image-iT FX signal enhancer for an improved signal-to-noise ratio

A pack of two CultureWell chambered coverglasses is also included for convenient processing. Each Image-iT FX Kit provides sufficient materials to perform 50–100 assays.

The Alexa Fluor dye conjugates are, without exception, the best conjugates we have ever tested. With excellent brightness and photostability, these fluorescent conjugates of streptavidin, goat anti–mouse, and goat anti–rabbit IgG are superior fluorescent secondary reagents for imaging.

• Learn more about Secondary Antibodies and Secondary Detection

ProLong Gold antifade reagent is premixed and ready to use—just add a drop to your preparation and mount. ProLong Gold reagent outperforms most other commercially available antifade reagents, significantly reducing fluorophore photobleaching while causing little or no quenching of the fluorescence signal. This reagent offers excellent compatibility with a multitude of dyes and dye complexes, making it an especially valuable tool for multicolor applications. ProLong Gold reagent cures within 24 hours, and the sample can be saved for months after mounting.

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ProLong Gold antifade reagent (top) provides significant photobleaching protection compared to PBS alone (bottom) over a 20-second time series. Images were acquired at 5-second intervals.

Image-iT FX signal enhancer dramatically improves the signal-to-noise ratio of immunolabeled cells and tissues, allowing you to clearly visualize targets that would normally be indistinguishable due to background fluorescence. Background staining typically seen with fluorescent dyes is largely eliminated when Image-iT FX signal enhancer is applied to fixed and permeabilized cells before staining (Table 1).

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Table 1. Fluorescent dyes successfully tested with the Image-iT FX Signal Enhancer.*

* All dyes were conjugated to streptavidin and tested at 10 µg/mL.