Cell morphology, working with live vs. fixed cells, and choosing a vessel
Before you approach the microscope, before you even begin to culture the cells you want to image, it's important to understand the aim of your experiment and how the cells you choose, the state they'll be in when you image them, and the vessel they're contained in will affect your ability to capture good images.
See guidelines for choosing a cell type to optimize the images you want to obtain
Learn the essential considerations for using live-cells in your imaging experiments
Understand the pros and cons of fixed-cell imaging and learn about the basic fixation treatments
See how various experimental and equipment parameters affect which vessel you should choose