Your go-to HPLC columns for separating complex mixtures
Thermo Scientific Acclaim HPLC Columns have a smaller pore size and higher surface area to deliver the high resolution you need for high-efficiency separations. Choose from a broad variety of functional groups including:
- Conventional reversed-phase C18 and polar embedded phases
- Mixed-mode phases
- Application-specific phases
Thermo Fisher Scientific designed and manufactured all Acclaim columns to tight specifications to ensure you get consistent performance.
Acclaim HPLC column families
| Column | Use for | Data sheet |
|---|---|---|
| Acclaim 120 C18 columns | High efficiency reversed-phase columns for small molecule and complex mixtures separations, including LC-MS | Acclaim 120 C18 data sheet |
| Acclaim 120 C8 columns | Reversed-phase columns for small molecule with intermediate hydrophobic retention | Acclaim 120 C8 data sheet |
| Acclaim 300 C18 columns | Wide pore columns for LC-MS life science applications; protein and peptides | Acclaim 300 C18 data sheet |
| Acclaim Polar Advantage (PA) columns | Polar-embedded reversed-phase columns for separating polar and nonpolar analytes with 100% aqueous mobile phase | Acclaim PA data sheet |
| Acclaim Polar Advantage (PA2) columns | Polar-embedded columns with enhanced hydrolytic stability pH 1.5 – 10, excellent for polar and non-polar analytes with 100% aqueous mobile phase and LC-MS | Acclaim PA2 data sheet |
| Acclaim C30 columns | Very high hydrophobic retention; good for separating structurally related isomers | Acclaim C30 data sheet |
| Acclaim HILIC-10 columns | Designed for unique selectivity of hydrophilic molecules |
| Specifications for Acclaim reversed-phase & general-purpose HPLC columns | ||||||||
|---|---|---|---|---|---|---|---|---|
| Acclaim 120 C18 | Acclaim 120 C8 | Acclaim 300 C18 | Acclaim PA | Acclaim PA2 | Acclaim C30 | Acclaim Phenyl-1 | Acclaim HILIC-10 | |
| Bonded Phase | Octadecylsilane | Octylsilane | Octadecylsilane | Embedded sulfonamide | Embedded Amide | C30 alkylsilane | Phenyl | Proprietary |
| USP Type | L1 | L7 | L1 | L60 | L60 | L62 | L11 | na |
| End-capped | Yes | Yes | Yes | Yes | Yes | Proprietary | Yes | |
| Starting Material | Ultrapure silica | Ultrapure silica | Ultrapure silica | Ultrapure silica | Ultrapure silica | Ultrapure silica | Ultrapure silica | Ultrapure silica |
| Particle Shape | Spherical | Spherical | Spherical | Spherical | Spherical | Spherical | Spherical | Spherical |
| Particle Sizes | 2.2 μm, 3 μm, 5 μm | 2.2 μm, 3 μm, 5 μm | 3 μm | 3 μm, 5 μm | 3 μm, 5 μm | 3 μm, 5 μm | 3 μm, 5 μm | 3 μm, 5 μm |
| Average Pore Diameter | 120 Å | 120 Å | 300 Å | 120 Å | 120 Å | 200 Å | 120 Å | 120 Å |
| Surface Area | 300 m2/g | 300 m2/g | 100 m2/g | 300 m2/g | 300 m2/g | 200 m2/g | 300 m2/g | 300 m2/g |
| Total Carbon Content | 18% | 11% | 7% | 17% | 17% | |||
| Column | Use for | Data sheet |
|---|---|---|
| Acclaim Trinity P1 columns |
| Acclaim Trinity P1 data sheet |
| Acclaim Trinity P2 columns |
| Acclaim Trinity P2 data sheet |
| Acclaim Mixed-Mode WAX-1 columns |
| Acclaim Mixed-Mode WAX-1 data sheet |
| Acclaim Mixed-Mode WCX-1 columns |
| Acclaim Mixed-Mode WCX-1 data sheet |
| Acclaim Mixed-Mode HILIC-1 columns |
| Acclaim Mixed-Mode HILIC-1 data sheet |
| Specifications for Acclaim mixed-mode HPLC columns | |||||
|---|---|---|---|---|---|
| Acclaim Trinity P1 | Acclaim Trinity P2 | Acclaim Mixed-Mode WCX-1 | Acclaim Mixed-Mode WAX-1 | Acclaim Mixed-Mode HILIC-1 | |
| Functionality | Reversed-phase + weak anion exchange + strong cation exchange | HILIC + strong anion exchange + weak cation exchange | Reversed-phase + weak cation exchange | reversed-phase + weak anion exchange | Reversed-phase + HILIC |
| USP Type | n.a. | n.a. | L85 | L78 | n.a. |
| Starting Material | Ultra-pure silica | Ultra-pure silica | Ultra-pure silica | Ultra-pure silica | Ultra-pure silica |
| Particle Shape | Spherical | Spherical | Spherical | Spherical | Spherical |
| Particle Sizes | 3 μm, 5 μm | 3 μm, 5 μm | 3 μm | 3 μm, 5 μm | 3 μm, 5 μm |
| Average Pore Diameter | 120 Å | 120 Å | 300 Å | 120 Å | 120 Å |
| Surface Area | 300 m2/g | 300 m2/g | 100 m2/g | 300 m2/g | 300 m2/g |
| Column | Use for | Data sheet |
|---|---|---|
| Acclaim Organic Acid HPLC Columns | Fast organic acid analysis; including both aromatic and aliphatic organic acids. | Acclaim Organic Acid data sheet |
| Acclaim Surfactant HPLC Columns | Designed for the separation of surfactants including anionic, non-ionic, cationic, and amphoteric surfactants in various matrices. The Surfactant Plus is compatible with LC-MS instrumentation | Acclaim Surfactant data sheet |
| Acclaim Surfactant Plus columns | Designed for the separation of surfactants including anionic, non-ionic, cationic, and amphoteric surfactants in various matrices. The Surfactant Plus column is compatible with LC-MS instrumentation | Acclaim Surfactant Plus data sheet |
| Acclaim Explosives E2 columns | Specialty columns to comply with EPA Method 8330; baseline resolution of all 14 explosive residues targeted by the EPA | |
| Acclaim Trinity Q1 columns | Excellent for trace analysis of diquat and paraquat | |
| Acclaim Carbonyl columns | Separation of DNPH derivatives of aldehydes and ketones; including regulated methods U.S. EPA 554, EPA 8315, EPA 1667, EPA TO-11, and CARB 1004. | Acclaim Carbonyl data sheet |
| Acclaim Carbamate columns | Separation of carbamate pesticides specified in U.S. EPA Method 531.2 | Acclaim Carbamate data sheet |
| Acclaim AmG C18 columns | Reversed-phase separation of aminoglycoside antibiotics. | Acclaim AmG C18 data sheet |
| Specifications for Acclaim application-specific columns | ||||||||
|---|---|---|---|---|---|---|---|---|
| Acclaim Organic Acid | Acclaim Surfactant | Acclaim Surfactant Plus | Acclaim Explosives E2 | Acclaim Carbonyl | Acclaim Carbamate | Acclaim AmG | Acclaim Trinity Q1 | |
| End-capped | Yes | Yes | Yes | Yes | Yes | Yes | Yes | Yes |
| Particle Shape | Spherical | Spherical | Spherical | Spherical | Spherical | Spherical | Spherical | Spherical |
| Particle Size | 3 μm, 5 μm | 3 μm, 5 μm | 3 μm, 5 μm | 2.2 μm, 3 μm, 5 μm | 2.2 μm, 3 μm, 5 μm | 2.2 μm, 3 μm, 5 μm | 3 μm | 3 μm, 5 μm |
| Average Pore Diameter | 120 Å | 120 Å | 120 Å | 300 Å | 120 Å | 120 Å | 300 Å | 300 Å |
| Surface Area | 300 m2/g | 300 m2/g | 300 m2/g | 100 m2/g | 300 m2/g | 300 m2/g | 100 m2/g | 100 m2/g |
| Column | Use for | Data sheet |
|---|---|---|
| Acclaim SEC-1000 and Acclaim SEC-300 | Separation of water-soluble polymers using resin based columns. | Acclaim SEC columns data sheet |
| Specifications for Acclaim size exclusion columns (SEC) | ||
|---|---|---|
| Acclaim SEC-300 | Acclaim SEC-1000 | |
| Substrate | Hydrophilic polymethacrylate resin | Hydrophilic polymethacrylate resin |
| Particle Shape | Spherical | Spherical |
| Particle Size | 5 μm | 7 μm |
| Pore Size | 300 Å (multi-pore) | 1000 Å (multi-pore) |
| Separation range for PEO* | 100 – 50,000 Daltons | 1,000 – 1,000,000 Daltons |
| Exclusion limit for PEO* | 50,000 – 150,000 Daltons | 3,000,000 – 7,500,000 Daltons |
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