Payload Selection and Transfection Reagents

Select the payload delivery technique you’re using:

Choose transfection solutions for DNA, RNA, mRNA, and protein delivery to best suit your experiments. Our products are designed to provide you with high transfection efficiency and cell viability.

Plasmid transfection

Select plasmid transfection products

Plasmid isolation
Transfection reagent

Plasmid miniprep, midiprep, maxiprep, megaprep, and gigaprep kits

Pure Link HiPure Plasmid Filter Maxiprep Kit

Low endotoxin (0.1–1 EU/µg)
Yield up to 20 µg–15 mg
Total protocol time of 30–120 min

ERROR processing Product List Table.
Skus Requested:
Skus Returned by ms service:

Find more plasmid purification kits

Lipofectamine 3000 reagent

Superior efficiency—10-fold higher efficiency into the broadest spectrum of difficult-to-transfect cells
Gentle with low toxicity—for improved cell viability
Best value—just pennies per reaction for best-in-class transfection results

ERROR processing Product List Table.
Skus Requested:
Skus Returned by ms service:

Find more reagents for plasmid DNA transfection

siRNA or miRNA transfection

Select siRNA or miRNA products

siRNA
miRNA
Transfection reagent

Silencer Select siRNAs

Up to 100-fold more potent than other available siRNAs and fewer off-target effects
Reduced off-target effects up to 90% due to locked nucleic acid (LNA) chemical modifications
100% guaranteed to silence, the best guarantee in the industry

ERROR processing Product List Table.
Skus Requested:
Skus Returned by ms service:

Find gene-specific siRNAs

mirVana miRNA mimics and inhibitors

mirVana miRNA mimic and inhibitor control

miRNA mimics

For microRNA gain-of-function studies
Highly specific due to chemical modifications that prevent passenger strand entry into RISC

miRNA inhibitors

For microRNA loss-of-function studies
Potent, long-lasting microRNA inhibition due to proprietary design and chemical modifications

Controls

ERROR processing Product List Table.
Skus Requested:
Skus Returned by ms service:

Find miRNA-specific mimics and inhibitors

For in vitro transfection

Lipofectamine RNAiMAX Reagent

Provides highly efficient siRNA/miRNA delivery with low cytotoxicity
Highly versatile across a wide range of cell types
Ideal for high-throughput siRNA/miRNA transfections

ERROR processing Product List Table.
Skus Requested:
Skus Returned by ms service:

For in vivo transfection

Invivofectamine 3.0 Transfection Reagent

Impressive results with up to 85% knockdown achieved using microgram levels of siRNA
Use up to 90% less siRNA than required with previous reagents for efficient, effective knockdown in vivo

ERROR processing Product List Table.
Skus Requested:
Skus Returned by ms service:

mRNA transfection

In vitro transcription
Transfection reagent

MEGAscript transcription kits

For synthesis of large amounts of unlabeled or low specific activity RNA
Yields over 10 times more RNA than conventional in vitro transcription reactions

ERROR processing Product List Table.
Skus Requested:
Skus Returned by ms service:

mMESSAGE mMACHINE transcription kits

mMESSAGE mMACHINE™ SP6 Transcription Kit

For synthesis of large amounts of capped RNA
Yields up to 10–50X conventional in vitro transcription reactions

ERROR processing Product List Table.
Skus Requested:
Skus Returned by ms service:

Find more in vitro transcription kits

Lipofectamine MessengerMAX Transfection Reagent

Lipofectamine MessengerMAX Reagent box and tube

Faster protein expression with no risk of genomic integration with mRNA-based transfection
Impressive mRNA delivery performance with >60% transfection efficiency in neurons and primary cells

ERROR processing Product List Table.
Skus Requested:
Skus Returned by ms service:

CRISPR-Cas9 transfection

Select DNA, RNA or Protein

DNA
RNA
Protein

GeneArt CRISPR-Cas9 vectors

GeneArt CRISPR Nuclease Vector with CD4 Enrichment Kit

All-in-one vector system for CRISPR-based genome editing
Affordable, ready-to-use cloning vectors
Provides enrichment for hard-to-transfect or difficult-to-modify cell lines

ERROR processing Product List Table.
Skus Requested:
Skus Returned by ms service:

Lipofectamine 3000 reagent

Superior efficiency—10-fold higher efficiency into the broadest spectrum of difficult-to-transfect cells
Gentle with low toxicity—for improved cell viability
Best value—just pennies per reaction for best-in-class transfection results

ERROR processing Product List Table.
Skus Requested:
Skus Returned by ms service:

TrueGuide synthetic guide RNA (sgRNA)

Illustration of TrueGuide Synthetic gRNA

Ready-to-transfect, chemically modified CRISPR sgRNAs
Designed by a proprietary algorithm for high-efficiency editing
Predesigned for knockout or create a custom design for precise gene editing

ERROR processing Product List Table.
Skus Requested:
Skus Returned by ms service:

Find catalog or custom synthetic gRNA

GeneArt CRISPR Nuclease mRNA

Ready to co-transfect with a target-specific guide RNA (gRNA)
Smaller payload than plasmid-based Cas9 systems for improved delivery into the cell and better genome editing efficiency
Can be used with more than one gRNA to edit multiple genomic loci with one transfection

ERROR processing Product List Table.
Skus Requested:
Skus Returned by ms service:

For transfection of sgRNA only to Cas9-expressing cells

Lipofectamine RNAiMAX Reagent

Provides highly efficient sgRNA delivery with low cytotoxicity
Highly versatile across a wide range of cell types
Ideal for high-throughput sgRNA transfections

ERROR processing Product List Table.
Skus Requested:
Skus Returned by ms service:

For co-transfection of sgRNA and Cas9 mRNA

Lipofectamine MessengerMAX Transfection Reagent

Faster protein expression with no risk of genomic integration
>60% transfection efficiency in neurons and primary cells

ERROR processing Product List Table.
Skus Requested:
Skus Returned by ms service:

TrueCut HiFi Cas9 Protein

Significantly reduce off-target effects in a broad range of cell types including primary, stem and immune cells
Preserved high on-target editing efficiency relative to TrueCut Cas9 v2 (wild type)

ERROR processing Product List Table.
Skus Requested:
Skus Returned by ms service:

Find more Cas9 proteins

Lipofectamine CRISPRMAX Cas9 Transfection Reagent

Provides highly efficient delivery of RNP (Cas9:gRNA complex) into cells with low toxicity
Demonstrated high cleavage efficiency in a broad range of cell types
Compatible with high-throughput setup

ERROR processing Product List Table.
Skus Requested:
Skus Returned by ms service:

Find more CRISPR gene editing products

Quick tip: Designing custom genome editing?

The Invitrogen TrueDesign Genome Editor is a free online tool that enables scientists of all experience levels to easily design, select, and order reagents for accurate and successful gene editing experiments.

Try TrueDesign Genome Editor

Cotransfection of DNA, RNA, protein

Lipofectamine 3000 reagent

Superior efficiency—10-fold higher efficiency into the broadest spectrum of difficult-to-transfect cells
Gentle with low toxicity—for improved cell viability
Best value—just pennies per reaction for best-in-class transfection results

ERROR processing Product List Table.
Skus Requested:
Skus Returned by ms service:

Performing electroporation?

Use the Neon Transfection System for a significant increase in transfection efficiency and cell viability.

Learn more

Invitrogen TransfectionSelect product selection tool

Pro tip

Find recommended transfection solutions, protocols, citations quickly with the TransfectionSelect product selection tool.

Try now

100 ml bottle of Opti-MEM I Reduced Serum Medium

Quick tip

Don’t forget Opti-MEM I Reduced Serum Medium to prepare the transfection complex.

ERROR processing Product List Table.
Skus Requested:
Skus Returned by ms service:

Need more information?

When selecting a transfection method, it is important to consider the payload you wish to deliver (DNA, RNA, or protein) and the type of cells you want to transfect.

Learn more about selecting the proper method and payload

For Research Use Only. Not for use in diagnostic procedures.