Invitrogen

Human PRPS2 Control Fragment Recombinant Protein

Name: Human PRPS2 Control Fragment Recombinant Protein
Brand: Invitrogen
SKU: RP-105432

Datasheet

Tech Support

Datasheet

Tech Support

Product Details

RP-105432

Applications

Tested Dilution

Publications

Control (Ctrl)

Assay-dependent

Blocking Assay (BLOCK)

Assay-dependent

Product Specifications

Species

Human

Expression System

E. coli

Amino acid sequence

GAKRYHAGYTLQISPSADWF

Tag

His-ABP-tag

Class

Recombinant

Type

Protein

Purity

>80% by SDS-PAGE and Coomassie blue staining

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

≥5.0 mg/mL

Purification

purified

Storage buffer

PBS/1M urea, pH 7.4

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Product Specific Information

Highest antigen sequence indentity to the following orthologs: Mouse (40%), Rat (40%).

This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-66936. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

Target Information

The Phosphoribosyl Pyrophosphate Synthetase 2 (PRPS2) gene plays a critical role in cellular metabolism by encoding an enzyme that conducts a key reaction linking central carbon metabolism and nucleotide production, essential for various biological functions. Structurally, PRPS2 shares a high sequence identity with PRPS1, making it less sensitive to allosteric inhibition and particularly significant in certain oncology contexts due to its upregulation at the translational level in some cancers. The enzyme encoded by PRPS2 is pivotal in coupling protein and nucleotide biosynthesis, aided by a unique cis-regulatory element in its 5' UTR, which is influenced by oncogenic pathways. This gene manifests a tissue-specific expression profile, predominantly in proliferative tissues such as the thymus, lung, and small intestine, highlighting its significance in cell growth and proliferation. The evolutionary origins of PRPS2, along with its functional diversification into multiple PRPS genes in eukaryotes, underscore its indispensable role in cellular physiology and pathology.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Phosphoribosyl pyrophosphate synthase II; PPRibP; PPRibP synthetase; PRS-II; ribose-phosphate diphosphokinase 2; Ribose-phosphate pyrophosphokinase 2; testicular secretory protein Li 41