Invitrogen

Human DDHD2 (aa 119-194) Control Fragment Recombinant Protein

Name: Human DDHD2 (aa 119-194) Control Fragment Recombinant Protein
Brand: Invitrogen
SKU: RP-93745

View all (2) DDHD2 proteins

Datasheet

Tech Support

Datasheet

Tech Support

Product Details

RP-93745

Applications

Tested Dilution

Publications

Control (Ctrl)

Assay-dependent

Blocking Assay (BLOCK)

Assay-dependent

Product Specifications

Species

Human

Expression System

E. coli

Amino acid sequence

GDKDNKYVPYSESFSQVLEETYMLAVTLDEWKKKLESPNREIIILHNPKLMVHYQPVAGSDDWGSTPTEQGRPRTV

Tag

His-ABP-tag

Class

Recombinant

Type

Protein

Purity

>80% by SDS-PAGE and Coomassie blue staining

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

≥5.0 mg/mL

Purification

purified

Storage buffer

PBS/1M urea, pH 7.4

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Product Specific Information

Highest antigen sequence indentity to the following orthologs: Mouse (91%), Rat (91%).

This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-54705. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

Target Information

DDHD2, also known as KIAA0725p, is a member of the intracellular phospholipase A1 (PLA1) protein family which comprises a group of enzymes that hydrolyze the sn-1 ester bond of phospholipids, producing 2-acyl-lysophospholipids and fatty acids. DDHD2 prefers phosphatidic acid as substrate and has a role in efficient membrane trafficking from the Golgi apparatus to the plasma membrane. The gene of DDHD2 maps to chromosome 8p11.23, and encodes a 711-amino-acid protein with a calculated molecular mass of 81 kDa. It has been reported that DDHD2 immunoblot analysis of various tissue extracts revealed two bands of 90 and 85 kDa.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DDHD domain-containing protein 2; intracellular phospholipase A1gamma; KIAA0725p; PA-PLA1 like; Phospholipase DDHD2; SAM, WWE and DDHD domain-containing protein 1; sec23p-interacting protein p125-like phosphatidic acid-preferring phospholipase A1; TAG hydrolase; Triacylglycerol hydrolase DDHD2; Triglyceride hydrolase DDHD2; Triglyceride lipase