">

Cell dissociation

Our cell dissociation reagents help you prepare a desired number of cells and plate them so that they are healthy, proliferating, and in a desired confluency at the time of transfection.

Select cell dissociation reagent type

For adherent cell culture, choose from trypsin and trypsin alternatives to dissociate cultured cells into single cells.

Trypsin-EDTA, phenol red

Trypsin with phenol red in 100 ml bottle
  • Fast cell dissociation
  • General purpose uses

Trypsin

Catalog # Name Size Price Qty
25300054 Trypsin-EDTA (0.05%), phenol red, 100 mL Each
38.39

25200056 Trypsin-EDTA (0.25%), phenol red, 100 mL Each
38.39

TrypLE Express Enzyme
100 mL TrysinLE Express bottle
  • Gentle, convenient
  • Animal origin-free
  • Inhibition by dilution (no inhibitors needed)

Trypsin alternative

ERROR processing Product List Table.
Skus Requested: 12605036
Skus Returned by ms service:
Balanced salt solutions
500 mL bottle of phosphate buffered saline (PBS)
  • High quality and consistency for reproducible results
  • Manufactured in cGMP, ISO-certified facilities
  • Tested for osmolality, pH, stability, bacterial and fungal contamination, and endotoxins

Wash solutions

Catalog # Name Size Price Qty
10010023 PBS, pH 7.4, 500 mL Each
77.00

14175095 HBSS, no calcium, no magnesium, no phenol red, 500 mL Each
81.00

Cell counting

Choose from manual and automated options to quickly and accurately count cells while minimizing user variation associated with counting.

Select manual or automated counting method

Trypan blue solution

100 mL bottle of 0.4% trypan blue stain
  • Determine cell viability quickly and accurately
  • Use with the hemocytometer or with the Countess chamber slides
Catalog # Name Size Price Qty
15250061 Trypan Blue Solution, 0.4% Each
51.00

The Invitrogen Countess 3 Automated Cell Counter uses advanced machine-learning image analysis algorithms to deliver accurate cell counts and viability in less than 30 seconds. Find disposable Countess Cell Counting Chamber Slide, the Countess Reusable Slide, stains and other accessories for Countess 3 Automated Cell Counter.

Countess 3 Automated Cell Counter
Pro tip

Use an automated cell counter to generate fast and highly accurate cell counts even for challenging cell sample types.

Learn more

Cell plating

Choose culture dishes, media, sera, and supplements to plate your cells and get them ready for transfection.

Select cell plating product type

Nunc Cell-Culture Treated Dishes and Plates

4-well, 6-well, 12-well, 24-well, and 48-well clear cell culture dishes
  • Multi-well plates with the Nunclon Delta surface treatment (proprietary energy source, not coating) for more hydrophilic surface and better cell attachment
  • Tested with at least four cell lines
BenchStable Cell Culture Media
500 mL BenchStable DMEM medium bottle with its packaging
  • Enable storage at room temperature for flexibility and convenience
  • Available in commonly used basal media formulations
  • High quality and consistency for results you can rely on
Catalog # Name Size Price Qty
A4192101 BenchStable™ DMEM, 500 mL EA 500 mL
77.00

A4192001 BenchStable™ DMEM/F12, 500 mL EA 500 mL
103.00

A4192201 BenchStable™ MEM, 500 mL EA 500 mL
68.00

A4192301 BenchStable™ RPMI 1640, 500 mL EA 500 mL
70.00

Fetal Bovine Serum, Premium
500 mL bottle of FBS certified US origin
  • Tested for up to 90 quality specifications, including 9 CFR virus testing, endotoxin, and performance
  • Consistent reliability, service, value, and innovation since 1962

Glutamine, glucose, pyruvate, etc.

100 ml bottle of 100X GlutaMAX-I
  • High quality, consistency, and performance from the most basic formulations to the newest innovations
Catalog # Name Size Price Qty
25030149 L-Glutamine (200 mM), 20 mL Each
20.61

35050061 GlutaMAX™ Supplement, 100 mL Each
73.00

A2494001 Glucose Solution EA 50 mL
56.00

11360070 Sodium Pyruvate (100 mM) Each
27.05

Need more information?

Successful transfection is influenced by many factors—the choice of the transfection method, health and viability of the cell line, number of passages, degree of confluency, quality and quantity of the nucleic acid used, and the presence or absence of serum in the medium can all play a part in the outcome of your transfection experiment. While it is possible to optimize specific transfection conditions to achieve high transfection efficiencies, it is important to note that some cell death is inevitable regardless of the transfection method used.

For Research Use Only. Not for use in diagnostic procedures.

">