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| 4 | 561/594 | Texas Red | 595 | 613 | (in buffer) 3 (in antifade) 3 | microscopy, flow cytometry, fluorescence microplate reader |
Invitrogen Texas Red dye is a bright red-fluorescent dye with excitation ideally suited to the 561 or 594 nm laser lines. It is commonly conjugated to antibodies and proteins for cellular imaging applications. Texas Red dye is extensively used to label protein conjugates such as phalloidins for cytoskeleton counterstaining or antibody conjugates for immunofluorescence.
Texas Red sulfonyl chloride is susceptible to hydrolysis and its low solubility in water may complicate its conjugation to some biomolecules. To overcome this difficulty, we offer Invitrogen Texas Red-X succinimidyl ester, which contains an additional seven-atom aminohexanoyl spacer ("X") between the fluorophore and its reactive group. Some conjugates are offered in the Texas Red-X formulation.
For brighter conjugates and greater photostability, we offer a Texas Red dye alternative—Alexa Fluor 594—as a reactive dye or conjugated to a variety of antibodies, peptides, proteins, tracers or amplification substrates.
A wide variety of Texas Red and Texas Red-X dye products, including antibodies and bioconjugates, are available that have been optimized for cellular labeling and detection. In addition, we offer reactive dyes that allows you to generate your own antibody conjugates or probes.
Protein Labeling Reagents Selection Guide (NHS ester, maleimide, etc.) Search Texas Red secondary antibodies
UV-irradiated muntjac skin fibroblasts labeled with Texas Red-X phalloidin. Fixed, permeabilized cells were stained with Texas Red-X phalloidin to visualize F-actin in the cytoskeleton. Endogenous biotin in the mitochondria was detected with Streptavidin, Alexa Fluor 488 Conjugate. Nuclei were counterstained with DAPI.