Fast, flexible, and spectrally brilliant—simplify high-speed, high-parameter analysis
- Save time per experiment, even for rare events—run samples up to 10x faster with acoustic-assisted focusing and acquisition speeds up to 35,000 events/sec. delivering reliable, high-quality data and easy detection of low-frequency populations without extra prep.
- Engineered to resist clogs—handle tough samples like tumors and sticky cells with minimal interruptions and smooth operation.
- Powerful optics with flexible detection modes—features 6 lasers, 51 fluorescent detectors, and 6 scatter channels—plus the ability to switch between spectral unmixing and conventional compensation on one system.
- Automated high-plex panel workflows—run 96- and 384-well plates with Invitrogen CytKick autosamplers and automate control acquisition for large, high-parameter workflows.
Fastest spectral flow cytometer in the industry
Maximize speed without compromising data quality. The Attune Xenith Flow Cytometer uses acoustic-assisted focusing to deliver high-speed, high-precision performance. Unlike traditional systems that utilize only hydrodynamic focusing, it maintains tight cell alignment even at flow rates up to 1,000 µL/min, helping ensure low CVs and reliable results—excellent for complex panels and rare event analysis. Run faster, reduce clogs, and boost productivity.
Time to acquire one million granulocytes at various flow rates
This table compares the time required to acquire one million granulocytes using traditional hydrodynamic-only flow cytometry versus the Attune Xenith Flow Cytometer with acoustic-assisted focusing. The Attune Xenith cytometer’s ability to operate at flow rates up to 1,000 µL/min enables dramatically faster acquisition—reducing run times from over an hour to just minutes. This high-throughput capability supports rare event detection, eliminates the need to concentrate challenging samples, and improves overall workflow efficiency. Combined with syringe-based, volumetric sample delivery and positive displacement fluidics, the Attune Xenith Flow Cytometer offers consistent, clog-resistant performance across a wide range of sample types and flow rates.
Time to acquire 1 million granulocytes: acoustic vs. traditional flow cytometry
| Instrument and flow rate | Time to 1 million granulocytes |
Relative rate |
|---|---|---|
Hydrodynamic focusing, 60 µL/min (all other systems in the industry) |
63 min 33 sec | − |
| Acoustic focusing, 200 µL/min | 13 min 20 sec | ~5x faster |
| Acoustic focusing, 500 µL/min | 5 min 47 sec | >10x faster |
| Acoustic focusing at 1,000 µL/min | 3 min 13 sec | 19.7x faster |
Figure 1. Up to 10X faster total workflow time with Attune Xenith Flow Cytometer. In a controlled, side-by-side comparison using a 10-color panel, the Attune Xenith Flow Cytometer cut total workflow time by up to 2-fold compared to leading spectral cytometers. The measured workflow included experiment setup, sample acquisition, spectral unmixing, and basic data analysis. Each sample included 20,000 events per single-stain control and 100,000 events for the fully stained sample, run at each vendor’s recommended flow rate. This time savings means faster data delivery, increased system availability, and more efficient use of lab resources.
Smarter fluidics. Fewer clogs. Better data
Working with tumor tissues, sticky primary cells, or complex immuno-oncology samples? The Attune Xenith Flow Cytometer is purpose-built to tackle your most clog-prone workflows. With innovative fluidics, real-time pressure sensing, and automated recovery, Attune Xenith Flow Cytometer doesn’t just resist clogs—it detects, protects, and recovers, keeping your data flowing and your samples safe.
How clog-resistant is the Attune Xenith cytometer?
The Attune Xenith Flow Cytometer is built to minimize clogs and maximize up time, even with challenging samples.
In internal tests (don’t try this yourself), it handled medium pulp orange juice—filtered and unfiltered—thanks to its robust fluidics design.
Easily detect rare event populations
With high-throughput acquisition, acoustic focusing, and advanced optics, Attune Xenith enables accurate rare event detection without needing to concentrate your samples. Maintain resolution, reduce prep time, and get statistically meaningful data—faster and more reliably, even in complex, high-parameter immunophenotyping panels. View the complete dataset in our application note.
Figure 2. Precision in rare event detection using a 25-color natural killer cell panel. The study utilized high sensitivity and specificity to interrogate natural killer (NK) cell populations, providing crucial insights for detailed research. Immature, mature and terminal NK populations were identified from human PBMCs, and surface marker expression of each of these subpopulations was characterized. The acquisition of rare events was achieved without the need to concentrate samples, while high sample throughput reduced time to results without compromising population resolution. The sensitivity in detection ensured robust data resolution, enabling the differentiation of even rare populations in high complexity panels. View complete data set here.
Versatility and flexibility
Figure 3. Equipped for both spectral unmixing and conventional compensation. This diagram highlights the optical configuration of the Attune Xenith Flow Cytometer, showcasing its six lasers (349, 405, 488, 561, 637, and 781 nm) and 51 fluorescent detectors with user-changeable filters—offering maximum flexibility in fluorophore selection and panel design. The system also includes six scatter detectors, including a violet scatter channel optimized for small particle detection and dual forward/side scatter off the blue laser for expanded detection range and polarization studies. Download the fluorophore and reagent selection guide to start building your next panel with confidence.
Dual-mode versatility for high-parameter flow
Not all flow cytometry experiments require spectral unmixing—and with the Attune Xenith Flow Cytometer, you don’t have to choose. This instrument offers dual-mode capability, enabling you to switch seamlessly between conventional compensation and spectral workflows on a single system.
Figure 4. Resolution of rare dendritic cell subsets in PBMCs across flow rates. Representative flow cytometry plots, using conventional compensation, demonstrating the resolution of dendritic cell (DC) subsets in human peripheral blood mononuclear cells (PBMCs) acquired on the Attune Xenith Flow Cytometer. A 20-color panel was used to identify classical myeloid DCs (cDCs), non-classical CD16⁺CD38⁺ myeloid DCs, and bi-variant plasmacytoid/myeloid DCs characterized by CD45RA and CD38 expression. Data were acquired across multiple flow rates, with consistent subset resolution maintained throughout, illustrating the system’s sensitivity, stability, and capacity for robust rare population detection. These results underscore the suitability of the platform for advanced immunophenotyping applications, particularly in rare event analysis. Download our latest 31color panel immunophenotyping poster.
Outpace traditional systems with powerful automation and remarkable speed
The Attune Xenith Flow Cytometer, paired with Invitrogen CytKick autosamplers, delivers our new standard in high-throughput spectral flow cytometry. Easily run 96- or 384-well plates with speed and precision, while automating control acquisition for high-parameter panels—cutting total run times and minimizing hands-on steps.
And now with automated shutdown, you can queue your final plate, walk away, and trust the system to handle cleanup and power-down on its own. No waiting around. No manual intervention. Just efficient, reliable performance—even when you’re not in the lab.
Figure 4. High-speed plate acquisition with automated end-of-run shutdown. In a side-by-side comparison of full 96-well plate runs (100 µL per well), the Attune Xenith Flow Cytometer outperformed leading spectral flow cytometers in total acquisition time. Data was collected using each system’s maximum recommended flow rate. The combination of acoustic focusing and CytKick autosampler enables significantly faster throughput—exceptional for high-volume environments like core facilities and large-scale studies.
Figure 5. Flow cytometric analysis of mouse spleen demonstrates consistent population resolution across flow rates. A 37-parameter panel was used to analyze single-cell suspensions prepared from mechanically dissociated and filtered (70 µm) mouse spleen tissue. Data acquisition at 200 and 1,000 µL/min shows preserved population resolution and clog-free operation on the Attune Xenith Flow Cytometer, enabling rapid analysis of CD19+ B cells and their subsets. Population resolution remained stable even at high event rates from concentrated samples, though sample dilution is recommended to minimize cellular aggregates while maximizing throughput. View the complete dataset in our latest poster.
Remote diagnostics
Onboard service cameras let you check flow cell cleanliness during QC and support remote troubleshooting—so issues can be resolved faster, often without a site visit. Real-time telemetry and system logging monitor key parameters like flow rate and pressure, enabling quicker diagnostics and smarter support. With a service-friendly design and built-in monitoring tools, the Attune Xenith Flow Cytometer reduces downtime and helps you stay focused on your research.
Figure 6. Live flow cell camera view. Visual inspection helps identify debris or buildup early, preventing issues that could impact data resolution and system performance.
Simple software, powerful analysis
Flowscape Software on the Attune Xenith Flow Cytometer simplifies high-parameter spectral flow cytometry with intuitive tools, smart automation, and seamless integration. Designed for both novice and expert users, it features a guided Spectral Setup Wizard, graphical thresholding, and automated compensation setup—streamlining complex workflows and improving reproducibility. Shared labs benefit from built-in role-based access control, time tracking, and automated billing. Plus, with free offline access and a unified interface shared with the Bigfoot Spectral Cell Sorter, Flowscape enables a consistent, flexible experience that helps researchers move from setup to discovery—faster. Access the software user manual here.
See what researchers are saying
“Attune Xenith Flow Cytometer is a robust and powerful instrument that allows our users to quickly set up assays in conventional or spectral mode with a lot of flexibility and the well-known reliability and high-speed that we know of the Attune line of products.”
Juan J. Garcia Vallejo, MD, PhD, MBA
Associate Professor, Molecular Cell Biology & Immunology (MCBI)
Director Microscopy and Cytometry Core Facility (MCCF)
“The Attune Xenith Flow Cytometer is a great spectral instrument with high-speed acquisition. Our core facility staff and research customers who are already familiar with the Attune NxT Flow Cytometer are able to expand their panels and transition seamlessly to the Attune Xenith, while using software that feels familiar. Researchers with experience on other spectral cytometry platforms in our facility are pleased with the increased speed and clog-resistance of Attune Xenith. Additionally, our staff benefit from the large fluid tanks, well-protected SIP, and walk-away shutdown that reduce the amount of daily maintenance time needed to keep the Attune Xenith ready for our researchers. The Attune Xenith is a good, solid cytometer platform capable of tackling a wide range of cytometry assays in one instrument. We feel it would be a welcome addition to a busy flow core.”
Kathryn Fox, PhD, SCYM(ASCP)CM - Flow Lab Technical Manager and Dagna Sheerar, SCYM(ASCP)CM - Flow Cytometry Director
University of Wisconsin Carbone Cancer Center
Flow Cytometry Laboratory
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Help with dyes and panels
Resources
This series of articles is intended to help you get started designing and executing experiments using spectral flow cytometry. Topics include an overview of the differences between conventional and spectral methods, panel design, control and sample preparation, panel evaluation, and data analysis.
For Research Use Only. Not for use in diagnostic procedures.