Explore cellular ultrastructure
Talos L120C G2 Transmission Electron Microscope
The Thermo Scientific Talos L120C G2 (S)TEM is a 20-120 kV thermionic (scanning) transmission electron microscope uniquely designed for performance and productivity. It’s useful across a wide range of samples and applications, such as 2D and 3D imaging of cells, cell organelles, asbestos, polymers, and soft materials, both at ambient and cryogenic temperatures. The Talos L120C allows you to acquire high-quality results with minimal effort, no matter your skill level. Routine 2D imaging of samples is facilitated by a simple and intuitive user interface (UI). By implementing fast and sophisticated automation in advanced 3D imaging workflows with superior TEM and STEM resolution for 20-120 kV instrumentation, it allows you to focus on scientific questions rather than microscope operation.
Gain insights into elemental composition
Characterize quality attributes of nanoparticles
Visualize 3D intracellular structures
Talos L120C (S)TEM) advantages
The Talos L120C takes imaging to the next level with the optional cryo-box and low-dose techniques, which facilitate imaging of samples preserved at cryogenic temperatures and produce high-quality images even for beam-sensitive materials. The cryo-box provides maximal protection of the cryo-specimen resulting in minimal ice growth contamination over long (>8 h) data collection sessions.
To enhance productivity and application flexibility, especially in multi-user and multi-application environments, the cryo-box is motorized and fully retractable to facilitate seamless switching between cryo-EM imaging, STEM imaging, and EDS analysis. The large pole-piece gap of the C-Twin lens provides high contrast and allows for high specimen tilts up to 90°, while the constant-power objective lens with low-hysteresis allows for straightforward and reproducible changes of imaging modes and high-tension settings in matter of seconds.
Overall, the Talos L120C (S)TEM combines application versatility with a reproducibly performing electron column to give you new opportunities for high-resolution 3D characterization, in situ dynamic observation with EDS and other diffraction applications, and a special emphasis on high-contrast imaging and cryo-TEM.
Talos L120C (S)TEM automation
Designed for multi-user and multi-discipline environments and equipped with a familiar UI shared across all Thermo Scientific TEM platforms, the Talos L120C TEM is ideal for novice and expert users alike. All daily TEM tunings have been automated to provide the best and most reproducible setup, easing the learning curve for novice operators and improving time-to-data for experienced users. The Talos L120C TEM also offers educational online help embedded within the UI. Additionally, Thermo Scientific application software packages facilitate intuitive settings and automated data collection for different use cases and workflows, such as large area 2D imaging, energy dispersive X-ray analysis, single particle analysis, MicroED and tomography.
Versatile multi-application system
Designed for routine TEM and STEM imaging and characterization of a variety of samples, including resin embedded cells, soft materials, nanoparticles, and protein complexes at ambient and cryo-conditions.
High quality images
High-contrast, high-quality TEM and STEM imaging by simultaneous, multiple signal detection with up to four-channel-integration STEM detectors.
Cryo-imaging
Retractable, motorized cryo-box and low-dose technique enable superior imaging of samples at cryogenic temperatures while preserving the possibility of STEM imaging and EDS analysis applications.
Chemical composition data
Flexible energy-dispersive X-ray spectroscopy (EDS) analysis for chemical information.
Space for more
Add tomography or in situ sample holders compatible with a large analytical pole piece gap allowing up to ±90° stage tilt range and ±375 µm stage height range.
Improved productivity and reproducibility
Ultra-stable column, SmartCam remote operation, and constant power objective lenses for quick mode and high-tension switches. Fast, easy switching for multi-user environments.
Auto-alignments
All daily TEM tunings—such as focus, Eucentric height, center beam shift, center condenser aperture, and rotation center—are automated.
Automated data collection
Automate single particle analysis imaging with Thermo Scientific EPU Software and tomography with Thermo Scientific Tomo 5 Software. Collect large area 2D images at different image resolutions using Thermo Scientific Maps Software with automatic image acquisition and stitching while documenting the overall context and entire area of the region of interest with exceptional quality.
Ceta CMOS Camera
The 4K × 4K Thermo Scientific Ceta CMOS Camera with large field of view enables live digital zooming with high sensitivity and high speed over the entire high-tension range. Specialized Ceta Cameras are available for low-dose imaging (Ceta-S and Ceta-F) and for MicroED (Ceta-D) applications.
Compact design
Smaller footprint and dimensions mean this tool can be accommodated into more challenging spaces while reducing infrastructure and support costs.
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2D thin section of positively stained muscle tissue. Magnification = 17,500x.
STEM thick-section image of yeast cells; sample courtesy of Wanda Kukulski, MRC LMB Cambridge, UK. Magnification = 35,000x.
Negative stain image of DNA origami particles; sample courtesy of Thomas Martin, MRC LMB Cambridge. Magnification = 57,000x.
Gold particles on Si3N4. Resolution < 1.0 nm.
High-resolution TEM Gold [100] and FFT.
Low-voltage TEM image showing morphology of a block copolymer. Sample courtesy of Kevin Jack and Idriss Blakey, The University of Queensland.
2D thin section of positively stained muscle tissue. Magnification = 17,500x.
STEM thick-section image of yeast cells; sample courtesy of Wanda Kukulski, MRC LMB Cambridge, UK. Magnification = 35,000x.
Negative stain image of DNA origami particles; sample courtesy of Thomas Martin, MRC LMB Cambridge. Magnification = 57,000x.
Gold particles on Si3N4. Resolution < 1.0 nm.
High-resolution TEM Gold [100] and FFT.
Low-voltage TEM image showing morphology of a block copolymer. Sample courtesy of Kevin Jack and Idriss Blakey, The University of Queensland.
Life Sciences techniques
2D TEM and STEM imaging
2D imaging using either the TEM or STEM techniques provides fast visualization of samples, such as resin embedded sections of physiological samples in pathology or purified nanoparticles and macromolecular assemblies. Imaging of large sample areas is possible using full automation of batch imaging of multiple regions of interest at different magnifications in MAPS software.
Particle characterization
Imaging of nanoparticles by negative stain TEM or cryo-TEM facilitates detailed characterization of samples in process development at the nanometer resolution range. The number of critical quality attributes can then be accurately assessed, such as particle size distribution, morphology, empty/full classification, or particle aggregation.
3D TEM and STEM tomography
3D electron tomography delivers structural information about pleiomorphic samples such as enveloped viruses or lipid nanoparticles. Spatial arrangements of intracellular compartments within the cell can be also determined using this technique.
2D TEM and STEM imaging
2D imaging using either the TEM or STEM techniques provides fast visualization of samples, such as resin embedded sections of physiological samples in pathology or purified nanoparticles and macromolecular assemblies. Imaging of large sample areas is possible using full automation of batch imaging of multiple regions of interest at different magnifications in MAPS software.
Particle characterization
Imaging of nanoparticles by negative stain TEM or cryo-TEM facilitates detailed characterization of samples in process development at the nanometer resolution range. The number of critical quality attributes can then be accurately assessed, such as particle size distribution, morphology, empty/full classification, or particle aggregation.
3D TEM and STEM tomography
3D electron tomography delivers structural information about pleiomorphic samples such as enveloped viruses or lipid nanoparticles. Spatial arrangements of intracellular compartments within the cell can be also determined using this technique.
Materials Science techniques
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