Invitrogen

Mouse Siglec-3 (CD33) Recombinant Protein

Name: Mouse Siglec-3 (CD33) Recombinant Protein
Brand: Invitrogen
SKU: RP-88211
Availability: InStock

View all (6) CD33 proteins

Datasheet

Tech Support

Datasheet

Tech Support

Testing Data (1)

Mouse Siglec-3 (CD33) Protein in SDS-PAGE (SDS-PAGE)

FIGURE: 1 / 1

Mouse Siglec-3 (CD33) Protein (RP-88211) in SDS-PAGE

SDS-PAGE analysis of Mouse Siglec-3 (CD33) (Product # RP-88211) shows purity to be: > 90 % (SDS-PAGE).

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Product Details

RP-88211

Applications

Tested Dilution

Publications

Control (Ctrl)

Assay-dependent

Product Specifications

Species

Mouse

Expression System

HEK293 cells

Amino acid sequence

Mouse CD33, amino acids Met1-Glu240 (Accession # NP_067268.1) with six amino acids (LEVLFQ) at the C-terminus

Molecular weight

25.6 kDa

Class

Recombinant

Type

Protein

Purity

>90% as determined by SDS-PAGE.

Endotoxin concentration

<1.0 EU/µg

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Storage buffer

PBS, pH 7.4, with 0.01% mannitol, 5-8% trehalose, 0.01% Tween 80

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

Product Specific Information

It is recommended that the protein be aliquoted for optimal storage.

It is recommended that sterile water (800 µL) be added to the vial to prepare a stock solution of 0.25 mg/mL.

Concentration is measured by UV.

Target Information

CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies, and these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia. Further, CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells. CD33 is also expressed on, and is a useful marker for, peripheral monocytes. CD33 is useful for distinguishing myelogenous leukemia cells from lymphoid or erythroid leukemias. Diseases associated with CD43 dysfunction include gallbladder lymphoma and extracutaneous mastocytoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CD33; FLJ00391; Myeloid cell surface antigen CD33; Sialic acid-binding Ig-like lectin 3; Siglec-3