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Product Details
PMC2044
Species
Expression System
Amino acid sequence
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Endotoxin concentration
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Conjugate
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Product Specific Information
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile, distilled water, to a concentration of 0.1-0.5 mg/mL. Do not vortex. This solution can be stored at 2°C to 8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein, such as 0.1% BSA and store in working aliquots at -80°C to -20°C.
Storage: The lyophilized protein is stable at room temperature for 1 month, but should be kept at -20°C for long term storage. Working aliquots stored with a carrier protein are stable for at least 3 months at -80°C to -20°C. Avoid repeated freeze/thaw cycles.
Target Information
M-CSF (Macrophage colony-stimulating factor, CSF-1) is a survival factor essential for the proliferation and development of monocytes, macrophages, and osteoclast progenitor cells. M-CSF also induces VEGF (vascular endothelial growth factor) secretion by macrophages, thereby mediating mobilization of endothelial progenitor cells and neovascularization. M-CSF is present as several bioactive isoforms that differ in potency and stability. The full-length protein is synthesized as a membrane-spanning protein that can be expressed on the cell surface or further cleaved and modified in the secretory vesicle. Further, M-CSF is a disulfide-bonded homodimer which is processed into one of two isoforms, a glycoprotein or a proteoglycan that has been modified by the addition of chondroitin sulfate to each subunit. Binding of M-CSF to its receptor, c-Fms (CSF-1R or CD115) induces dimerization of the receptor followed by internalization and degradation of the complex. Functionally, M-CSF is known to stimulate differentiation of hematopoietic stem cells to monocyte-macrophage cell populations in culture. M-CSF acts through the CSF receptor 1. Although human M-CSF shows activity on mouse cells, mouse CSF shows no activity on human cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Bioinformatics
Protein Aliases: CSF-1; H-MCSF; M-MCSF; Macrophage colony-stimulating factor 1; MGC31930; osteopetrosis; PG-M-CSF; Proteoglycan macrophage colony-stimulating factor
Gene Aliases: C87615; Csf1; Csfm; MCSF; op
UniProt ID: (Mouse) P07141
Entrez Gene ID: (Mouse) 12977
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