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  • TLE3 Proteins

Invitrogen

Human TLE3 (aa 173-215) Control Fragment Recombinant Protein

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Datasheet
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Datasheet
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Cite Human TLE3 (aa 173-215) Control Fragment Recombinant Protein

Product Details

RP-101321

Applications
Tested Dilution
Publications

Control (Ctrl)

Assay-dependent
-

Blocking Assay (BLOCK)

Assay-dependent
-
Product Specifications

Species

Human

Expression System

E. coli

Amino acid sequence

GSQAHLTVKDEKNHHELDHRERESSANNSVSPSESLRASEKHR

Tag

His-ABP-tag

Class

Recombinant

Type

Protein

Purity

>80% by SDS-PAGE and Coomassie blue staining

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

≥5.0 mg/mL

Purification

purified

Storage buffer

1M urea/PBS, pH 7.4

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Product Specific Information

Highest antigen sequence indentity to the following orthologs: Mouse (95%), Rat (95%).

This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-62924. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

Target Information

The TLE genes are differentially expressed and encode nuclear proteins, consistent with the presence of sequence motifs associated with nuclear functions. These genes are the human homologues of Drosophila groucho, a member of the Notch signaling pathway which controls a number of different cell-fate choices in invertebrates and vertebrates.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: enhancer of split groucho 3; Enhancer of split groucho-like protein 3; ESG-1; ESG-2; ESG-3; ESG-4; ESG3; Tle1; Tle2; tle4; transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila); transducin-like enhancer of split 3 short isoform; transducin-like enhancer of split 3, homolog of Drosophila E(sp1); Transducin-like enhancer protein 3

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Gene Aliases: ESG; ESG3; GRG3; HsT18976; KIAA1547; TLE3

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UniProt ID: (Human) Q04726

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Entrez Gene ID: (Human) 7090

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It has to be done as per old AB suggested Products section.
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