Invitrogen

Human TIRAP (aa 79-162) Control Fragment Recombinant Protein

Name: Human TIRAP (aa 79-162) Control Fragment Recombinant Protein
Brand: Invitrogen
SKU: RP-97392

View all (3) TIRAP proteins

Datasheet

Tech Support

Datasheet

Tech Support

Product Details

RP-97392

Applications

Tested Dilution

Publications

Control (Ctrl)

Assay-dependent

Blocking Assay (BLOCK)

Assay-dependent

Product Specifications

Species

Human

Expression System

E. coli

Amino acid sequence

SSRWSKDYDVCVCHSEEDLVAAQDLVSYLEGSTASLRCFLQLRDATPGGAIVSELCQALSSSHCRVLLITPGFLQDPWCKYQML

Tag

His-ABP-tag

Class

Recombinant

Type

Protein

Purity

>80% by SDS-PAGE and Coomassie blue staining

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

≥5.0 mg/mL

Purification

purified

Storage buffer

PBS/1M urea, pH 7.4

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Product Specific Information

Highest antigen sequence indentity to the following orthologs: Mouse (92%), Rat (92%).

This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-111393. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

Target Information

Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. These proteins act through adaptor molecules such as TIRAP and MyD88 to activate various kinases and transcription factors. In TIRAP-deficient mice, TLR signaling in response to TLR2 ligands (using either TLR1 and TLR6 as co-receptors) is totally abolished, suggesting that MyD88 and TIRAP work together and are both required for TLR2 signaling. Furthermore, these mice are also resistant to the toxic effects of LPS and show defects in NF-βB and MAP kinase activation, suggesting that TIRAP is also involed in TLR4 signaling.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: adapter protein wyatt; Adaptor protein Wyatt; FLJ42305; HGNC:17192; MyD88 adapter-like protein; MyD88-2; OTTHUMP00000179130; TIR domain-containing adapter protein; toll-interleukin 1 receptor (TIR) domain containing adaptor protein; Toll-like receptor adaptor protein; Toll/interleukin-1 receptor domain-containing adapter protein