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Invitrogen
Human THEM4 (aa 147-239) Control Fragment Recombinant Protein
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Product Details
RP-94335
Species
Expression System
Amino acid sequence
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Conjugate
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Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (70%), Rat (70%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-55781. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
THEM4, also known as CTMP, binds specifically to the carboxy-terminal regulatory domain of PKB/Akt at the plasma membrane and acts as a negative regulator, reversing the phenotype of v-Akt-transformed cells. Hypermethylation of the THEM4 promoter and transcriptional downregulation of the gene has been reported in multiple glioblastomas, suggesting that epigenetic regulation of THEM4 may play a role in the progression of this cancer. Bioinformatic analysis, confirmed by in vitro testing, indicates that THEM4 is a broad-range, high activity acyl-CoA thioesterase. Recent reports have also indicated that TMEM4 is a mitochondrial protein whose overexpression is associated with an increase in mitochondrial membrane depolarization and caspase-3 and PARP cleavage, suggesting that THEM4 is involved in the apoptotic program.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Bioinformatics
Protein Aliases: Acyl-CoA thioesterase THEM4; Acyl-coenzyme A thioesterase THEM4; C-terminal modulator protein; Carboxyl-terminal modulator protein; Thioesterase superfamily member 4
Gene Aliases: CTMP; THEM4
UniProt ID: (Human) Q5T1C6
Entrez Gene ID: (Human) 117145
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