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Human TGF-beta 1 Protein (PHG9214) in Functional
Product Details
PHG9214
Species
Published species
Expression System
Amino acid sequence
Molecular weight
Class
Type
Purity
Endotoxin concentration
Activity
Conjugate
Form
Purification
Storage buffer
Contains
Storage conditions
Shipping conditions
Product Specific Information
Reconstitution: Centrifuge the vial briefly, before opening to bring the contents to the bottom. Reconstitute the lyophilized protein in an acidic buffer, such as 40 mM acetic acid or 4 mM HCl, to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For long term storage, it is recommended to further dilute in buffer containing a carrier protein, such as 0.1% BSA, and store in working aliquots at -20°C to -80°C.
Storage: Store the lyophilized protein at 2-8°C or -20°C, preferably desiccated. Store reconstituted human TGF-beta 1 at ≤-20°C (not in a frost-free freezer). Avoid repeated freeze-thaw cycles.
Target Information
TGF beta-1 (TGFB1, Transforming Growth Factor Beta 1) is a polypeptide member of the transforming growth factor beta superfamily of cytokines, found almost ubiquitously in tissues. Transforming growth factor (TGF)-b is stored in the extracellular matrix as a latent complex with its pro-domain. Activation of TGF beta-1 requires the binding of aV integrin to an RGD sequence in the prodomain and exertion of force on this domain, which is held in the extracellular matrix by latent TGF-b binding proteins. Latent forms are complexes of TGF beta-1, an amino-terminal portion of the TGF-beta precursor, designated TGF-LAP (TGF-latency associated peptide), and a specific binding protein, known as LTBP. TGF beta-1 helps regulates proliferation, differentiation, adhesion, migration in many cell types. Many cells have TGF beta receptors, and the protein positively and negatively regulates many other growth factors. TGF beta-1 is cleaved into a latency-associated peptide and a mature TGF beta-1 peptide, and is found in either a latent form composed of a TGFB1 homodimer, a LAP homodimer, and a latent TGFB1-binding protein, or in an active form composed of a TGF beta-1 homodimer. The mature peptide may also form heterodimers with other TGF beta family members. The gene for TGF beta-1 is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease and cystic fibrosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: latency-associated peptide; prepro-transforming growth factor beta-1; TGF b; TGF b 1; TGF beta; tgf beta 1; TGF beta-1; TGF beta-1 protein; tgf beta1; TGF β; TGF β 1; TGF β1; TGF-B1; TGF-beta 1 protein; TGF-beta-1; TGFβ1; Transforming growth factor; transforming growth factor b1; transforming growth factor beta; Transforming growth factor beta-1 proprotein; transforming growth factor β1
Gene Aliases: CED; DPD1; LAP; TGFB; TGFB1; TGFbeta
UniProt ID: (Human) P01137
Entrez Gene ID: (Human) 7040
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