Invitrogen

Human RhoV (aa 195-236) Control Fragment Recombinant Protein

Name: Human RhoV (aa 195-236) Control Fragment Recombinant Protein
Brand: Invitrogen
SKU: RP-104211
Availability: InStock

Datasheet

Tech Support

Datasheet

Tech Support

Product Details

RP-104211

Applications

Tested Dilution

Publications

Control (Ctrl)

Assay-dependent

Blocking Assay (BLOCK)

Assay-dependent

Product Specifications

Species

Human

Expression System

E. coli

Amino acid sequence

KEVFDSAILSAIEHKARLEKKLNAKGVRTLSRCRWKKFFCFV

Tag

His-ABP-tag

Class

Recombinant

Type

Protein

Purity

>80% by SDS-PAGE and Coomassie blue staining

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

≥5.0 mg/mL

Purification

purified

Storage buffer

PBS/1M urea, pH 7.4

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Product Specific Information

Highest antigen sequence indentity to the following orthologs: Mouse (100%), Rat (100%).

This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-62781. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

Target Information

The Rho subfamily of Ras-related GTPases controls multiple aspects of cell function, including cytoskeletal rearrangement, nuclear signaling and cell growth. Rho V (ras homolog gene family, member V), also known as Rho GTPase-like protein ARHV, CHP or WRCH2 (Wnt-1 responsive Cdc42 homolog 2), is a 236 amino acid protein that controls the actin cytoskeleton through activation of the JNK pathway. A member of the Rho family and small GTPase superfamily, Rho V functions as a lipid anchor at the cytoplasmic side of the cell membrane and is expressed in placenta, pancreas and fetal brain. Rho V is implicated in cell transformation and is encoded by a gene located on human chromosome 15, which houses over 700 genes and comprises nearly 3% of the human genome. Angelman syndrome, Prader-Willi syndrome, Tay-Sachs disease and Marfan syndrome are all associated with defects in chromosome 15-localized genes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CDC42-like GTPase 2; GTP-binding protein-like 2; ras homolog gene family, member V; Rho GTPase-like protein ARHV; Rho-related GTP-binding protein RhoV; RHOV; Wnt-1 regulated Cdc42 homolog 2; Wnt-1 responsive Cdc42 homolog 2; WRCH-2; WRCH1-related GTPase