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  • PYCR1 Proteins

Invitrogen

Human PYCR1 (aa 283-316) Control Fragment Recombinant Protein

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Datasheet
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Datasheet
Tech Support

Cite Human PYCR1 (aa 283-316) Control Fragment Recombinant Protein

Product Details

RP-100259

Applications
Tested Dilution
Publications

Control (Ctrl)

Assay-dependent
-

Blocking Assay (BLOCK)

Assay-dependent
-
Product Specifications

Species

Human

Expression System

E. coli

Amino acid sequence

KKTILDKDHLPLELGSPEGLHPLLLQYQLARAPS

Tag

His-ABP-tag

Class

Recombinant

Type

Protein

Purity

>80% by SDS-PAGE and Coomassie blue staining

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

≥5.0 mg/mL

Purification

purified

Storage buffer

1M urea/PBS, pH 7.4

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Product Specific Information

Highest antigen sequence indentity to the following orthologs: Mouse (45%), Rat (45%).

This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-61565. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

Target Information

This gene encodes an enzyme that catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. The protein forms a homopolymer and localizes to the mitochondrion. Alternate splicing results in two transcript variants encoding different isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: P5C reductase 1; proliferation-inducing protein 45; Pyrroline-5-carboxylate reductase 1, mitochondrial

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Gene Aliases: ARCL2B; ARCL3B; P5C; P5CR; PIG45; PP222; PRO3; PYCR; PYCR1

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UniProt ID: (Human) P32322

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Entrez Gene ID: (Human) 5831

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It has to be done as per old AB suggested Products section.
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