Invitrogen

Human POLR2H (aa 2-75) Control Fragment Recombinant Protein

Name: Human POLR2H (aa 2-75) Control Fragment Recombinant Protein
Brand: Invitrogen
SKU: RP-96417

View all (2) POLR2H proteins

Datasheet

Tech Support

Datasheet

Tech Support

Product Details

RP-96417

Applications

Tested Dilution

Publications

Control (Ctrl)

Assay-dependent

Blocking Assay (BLOCK)

Assay-dependent

Product Specifications

Species

Human

Expression System

E. coli

Amino acid sequence

AGILFEDIFDVKDIDPEGKKFDRVSRLHCESESFKMDLILDVNIQIYPVDLGDKFRLVIASTLYEDGTLDDGEY

Tag

His-ABP-tag

Class

Recombinant

Type

Protein

Purity

>80% by SDS-PAGE and Coomassie blue staining

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

≥5.0 mg/mL

Purification

purified

Storage buffer

1M urea/PBS, pH 7.4

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Product Specific Information

Highest antigen sequence indentity to the following orthologs: Mouse (100%), Rat (100%).

This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-58085. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

Target Information

Eukaryotes produce 3 distinct classes of RNA polymerase, Pol I, II and III. Each polymerase is responsible for the synthesis of a different class of RNA. RNA polymerase I (Pol I) transcribes the rRNA (ribosomal RNA) genes for the precursor of the 28S, 18S, and 5. 8S molecules of the ribosome. RNA polymerase II transcribes protein-encoding genes into mRNA (messenger RNA) and snRNA (small nuclear RNA) genes into snRNAs that influence the processing of other classes of RNA. RNA polymerase III (Pol III) transcribes the 5S rRNA genes and all of the tRNA (transfer RNA) genes. Each class of RNA polymerase is assembled from 9 to 15 different polypeptides. The RPB6 and RPB8 subunits are shared by all 3 RNA polymerases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ABC3; DNA-directed RNA polymerase II subunit H; DNA-directed RNA polymerases I, II, and III 17.1 kDa polypeptide; DNA-directed RNA polymerases I, II, and III subunit RPABC3; hRPB8; hsRPB8; polymerase (RNA) II (DNA directed) polypeptide H; polymerase (RNA) II subunit H; RNA polymerases I, II, and III subunit ABC3; RPB17; RPB8 homolog