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Invitrogen
Human POLB (aa 266-335) Control Fragment Recombinant Protein
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Product Details
RP-103044
Species
Expression System
Amino acid sequence
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Type
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Conjugate
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Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (97%), Rat (97%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-83950. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
DNA polymerase beta comprises an amino-terminal 8- kDa domain and a carboxy-terminal 31- kDa domain. The N-terminal ssDNA binding domain has a deoxyribose phosphodiesterase activity while the C-terminal domain has a nucleotidyltransferase activity. Mammalian DNA polymerase beta, a DNA repair polymerase, is constitutively expressed in cultured cells, but treatment of cells with the DNA-alkylating agents such as N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) or methyl methanesulfonate up-regulates beta-pol level. DNA polymerase beta fills single nucleotide gaps in DNA produced by the base excision repair pathway of mammalian cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Bioinformatics
Protein Aliases: 5'-deoxyribose-phosphate lyase; 5'-dRP lyase; AP lyase; DNA pol beta; DNA polymerase beta; DNA polymerase beta subunit; MGC125976; POL B; polymerase (DNA directed), beta; polymerase (DNA) beta; polymerase B
Gene Aliases: POLB
UniProt ID: (Human) P06746
Entrez Gene ID: (Human) 5423
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