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Invitrogen
Human PIK3CA E545K/PIK3R1 (p110 alpha E545K/p85 alpha), His Tag Recombinant Protein
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Product Details
A31083
Species
Expression System
Amino acid sequence
Tag
Molecular weight
Class
Type
Conjugate
Form
Concentration
Purification
Storage buffer
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Product Specific Information
For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 µg, 10 µg and 20 µg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 µL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase.
This PIK3CA E545K/PIK3R1 (p110 alpha E545K/p85 alpha) recombinant human protein (full length) was expressed in insect cells.
PIK3CA E545K/PIK3R1 (p110 alpha E545K/p85 alpha) recombinant human protein is also available in our SelectScreen™ Kinase Profiling Service.
• Validated for use with the Adapta™ assay
• Available in multiple pack sizes for convenient use
• Also available in our SelectScreen Kinase Profiling Service
Protein Form: Full Length, Recombinant, Active, Mutant
The gene accession number for PIK3CA (p110α) is NP_006209.2 and for PIK3R1 (p85α) is NP_852664.1.
Target Information
PIK3CA E542K/PIK3R1 (p110 alpha E542K/p85 alpha) lipid kinase is comprised of a 110 kDa catalytic subunit and an 85 kDa regulatory subunit. A number of isoforms of the 110 kDa catalytic subunit and the 85 kDa regulatory subunit exist in cells. PIK3CA is frequently mutated or amplified in a variety of cancers including ovarian and colon and this kinase is one of the PI3K mutants. PIK3CA gene copy number is increased in over 30% of ovarian cancers and this leads to increased PI3-kinase activity. Furthermore, the activity of PIK3CA is essential for vascular development and inactivation of PIK3CA leads to severe defects in angiogenic sprouting and vascular remodeling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Bioinformatics
Protein Aliases: Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; Phosphatidylinositol 3-kinase regulatory subunit alpha; phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha; phosphatidylinositol 3-kinase, catalytic, alpha polypeptide; phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha); phosphatidylinositol 3-kinase-associated p-85 alpha; Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha; Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform; phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit, alpha isoform; Phosphoinositide 3-kinase alpha; Phosphoinositide-3-kinase catalytic alpha polypeptide; phosphoinositide-3-kinase regulatory subunit; phosphoinositide-3-kinase regulatory subunit alpha; phosphoinositide-3-kinase, catalytic, alpha polypeptide; phosphoinositide-3-kinase, regulatory subunit 1 (alpha); PI3-kinase p110 subunit alpha; PI3-kinase regulatory subunit alpha; PI3-kinase subunit alpha; PI3-kinase subunit p85-alpha; PI3K regulatory subunit alpha; PIK3CA; PIK3R1; ptdIns-3-kinase regulatory subunit alpha; PtdIns-3-kinase subunit p110-alpha; Serine/threonine protein kinase PIK3CA
Gene Aliases: AGM7; CLOVE; CWS5; GRB1; IMD36; MCAP; MCM; MCMTC; p110-alpha; p85; p85-ALPHA; PI3K; PI3K-alpha; PIK3CA; PIK3R1
UniProt ID: (Human) P42336, (Human) P27986
Entrez Gene ID: (Human) 5290, (Human) 5295
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