Invitrogen

Human NGF Recombinant Protein

Name: Human NGF Recombinant Protein
Brand: Invitrogen
SKU: RP-88190
Availability: InStock

View all (11) NGF proteins

Datasheet

Tech Support

Datasheet

Tech Support

Testing Data (1)

Human NGF Protein in SDS-PAGE (SDS-PAGE)

FIGURE: 1 / 1

Human NGF Protein (RP-88190) in SDS-PAGE

SDS-PAGE analysis of Human NGF (Product # RP-88190). The specification for purity shows: ≥ 95 % (SDS-PAGE), ≥ 90 % (SEC-HPLC), ≥ 90% (SEC-MALS, routine testing).

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Product Details

RP-88190

Applications

Tested Dilution

Publications

Functional Assay (Functional)

Assay-dependent

Product Specifications

Species

Human

Expression System

CHO cells

Amino acid sequence

Mature form of human beta-NGF, amino acids Ser122-Arg239 (Accession # NP_002497.2)

Molecular weight

13.2 kDa

Class

Recombinant

Type

Protein

Purity

>95% as determined by SDS-PAGE.

Endotoxin concentration

<1.0 EU/µg

Activity

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.2-2 ng/mL.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Storage buffer

40mM arginine/40mM histidine, pH 5.5, with 5-8% mannitol, 150mM NaCl, 5-8% trehalose, 0.01% Tween 80

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

Product Specific Information

It is recommended that the protein be aliquoted for optimal storage.

It is recommended that sterile water (350 µL) be added to the vial to prepare a stock solution of 0.25 mg/mL.

Concentration is measured by UV.

Target Information

Nerve growth factor (NGF) influences the survival and differentiation of a variety of neuronal and nonneuronal cells. The actions of this polypeptide result from binding to specific cell surface receptors which are present as both high and low affinity sites. Although both types of receptor bind NGF only the high affinity form is capable of signal transduction. Cross-linking studies with 125I-NGF have shown that the high affinity receptor of rat PC12 cells forms an Mr 158,000 complex with NGF while the low affinity receptor forms an Mr 100,000 complex. Full length cDNA clones for both the human and rat NGF receptor genes have been produced and sequenced, revealing greater than 90% homology between the two species. NGF exhibits nerve growth stimulating activity and is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Further, NGF stimulates division and differentiation of sympathetic and embryonic sensory neurons. Mutations in the NGF gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of NGF gene expression is associated with allergic rhinitis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: B nerve growth factor; b-NGF; beta ngf; Beta-nerve growth factor; Beta-NGF; BetaNGF; bNGF; MGC161426; MGC161428; nerve growth factor (beta polypeptide); nerve growth factor, beta subunit; NGF b; NGF β; NGFbeta; NGFβ; β nerve growth factor; β NGF; βNGF