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  • MRLC2 Proteins

Invitrogen

Human MRLC2 (aa 143-172) Control Fragment Recombinant Protein

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Datasheet
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Datasheet
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Cite Human MRLC2 (aa 143-172) Control Fragment Recombinant Protein

Product Details

RP-104383

Applications
Tested Dilution
Publications

Control (Ctrl)

Assay-dependent
-

Blocking Assay (BLOCK)

Assay-dependent
-
Product Specifications

Species

Human

Expression System

E. coli

Amino acid sequence

YREAPIDKKGNFNYIEFTRILKHGAKDKDD

Tag

His-ABP-tag

Class

Recombinant

Type

Protein

Purity

>80% by SDS-PAGE and Coomassie blue staining

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

≥5.0 mg/mL

Purification

purified

Storage buffer

1M urea/PBS, pH 7.4

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Product Specific Information

Highest antigen sequence indentity to the following orthologs: Mouse (100%), Rat (100%).

This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-83767. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

Target Information

MRLC2 is involved in the the activity of nonmuscle myosin II is regulated by phosphorylation of a regulatory light chain. This phosphorylation results in higher MgATPase activity and the assembly of myosin II filaments.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: MLC-2; MLC-2A; MLC20; MLCB; MRLC3; Myosin regulatory light chain 12B; myosin regulatory light chain 2; Myosin regulatory light chain 2-B, smooth muscle isoform; Myosin regulatory light chain 20 kDa; Myosin regulatory light chain MRLC2; myosin, light chain 12B, regulatory; RLC; SHUJUN-1

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Gene Aliases: MLC-B; MRLC2; MYL12B; MYLC2B

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UniProt ID: (Human) O14950

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Entrez Gene ID: (Human) 103910

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It has to be done as per old AB suggested Products section.
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