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  • MARCH10 Proteins

Invitrogen

Human MARCH10 (aa 379-475) Control Fragment Recombinant Protein

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Datasheet
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Datasheet
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Cite Human MARCH10 (aa 379-475) Control Fragment Recombinant Protein

Product Details

RP-93424

Applications
Tested Dilution
Publications

Control (Ctrl)

Assay-dependent
-

Blocking Assay (BLOCK)

Assay-dependent
-
Product Specifications

Species

Human

Expression System

E. coli

Amino acid sequence

LPDRESATEKDRGGSENAKKSPLSWDTKSEPRQEVGVNAENVWSDCISVEHRPGTHDSEGYWKDYLNSSQNSLDYFISGRPISPRSSVNSSYNPPAS

Tag

His-ABP-tag

Class

Recombinant

Type

Protein

Purity

>80% by SDS-PAGE and Coomassie blue staining

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

≥5.0 mg/mL

Purification

purified

Storage buffer

1M urea/PBS, pH 7.4

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Product Specific Information

Highest antigen sequence indentity to the following orthologs: Mouse (32%), Rat (32%).

This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-54763. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

Target Information

MARCH10 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 10-Mar; FLJ35757; MARCH-X; membrane associated ring finger 10; membrane-associated ring finger (C3HC4) 10, E3 ubiquitin protein ligase; Membrane-associated RING finger protein 10; Membrane-associated RING-CH protein X; Probable E3 ubiquitin-protein ligase MARCHF10; RING finger protein 190; RING-type E3 ubiquitin transferase MARCHF10; testis secretory sperm-binding protein Li 228n

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Gene Aliases: MARCH-X; MARCH10; MARCHF10; RNF190

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UniProt ID: (Human) Q8NA82

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Entrez Gene ID: (Human) 162333

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It has to be done as per old AB suggested Products section.
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