Invitrogen

Human IL-12 p35 Recombinant Protein

Name: Human IL-12 p35 Recombinant Protein
Brand: Invitrogen
SKU: RP-75615

Datasheet

Tech Support

Datasheet

Tech Support

Product Details

RP-75615

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

Control (Ctrl)

Assay-dependent

Product Specifications

Species

Human

Expression System

E. coli

Amino acid sequence

Amino acids Arg23- Ser219 containing an N-terminal His-tag

Tag

His-tag

Class

Recombinant

Type

Protein

Purity

>95%, by SDS-PAGE under reducing conditions

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.19 mg/mL

Purification

purified

Storage buffer

20mM tris HCl, pH 8, with 50% glycerol, 1mM EDTA

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

Product Specific Information

This product contains purified recombinant human IL-12 p35 protein with an N-terminal hexahistidine tag.

This product may be used as a positive control in many immunological assays.

Target Information

Interleukin 12A is a novel signaling cytokine belonging to the Interleukin-6 protein superfamily. Interleukin-12 alpha chain precursor (IL-12A) is a 35-kD subunit of disulfide-linked heterodimer of cytokine IL-12 and has a broad array of biological activities. Reports suggest that IL-12A can act as a growth factor for activated T and NK cells and enhance the lytic activity of NK/lymphokine-activated Killer cells. IL-12A stimulates the production of IFN-gamma by resting PBMC as well as T-cell-independent induction of interferon (IFN)-gamma, and helps in the differentiation of both Th1 and Th2 cells. It is also involved in positive regulation of tyrosine phosphorylation of Stat4 protein. The signaling process of IL-12A in innate immunity requires nitric oxide synthase 2A. Along with EBI3, IL-12A forms a novel heterodimeric cytokine, designated as interleukin-35 (IL-35), that may be specifically produced by T reg cells and is required for maximal suppressive activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CLMF p35; cytotoxic lymphocyte maturation factor 1, p35; Cytotoxic lymphocyte maturation factor 35 kDa subunit; il 12; IL-12 subunit p35; IL-12, subunit p35; IL-12A; IL12p35; IL35 subunit; ILN; Interleukin; Interleukin 12; interleukin 12, p35; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); interleukin-12 alpha chain; Interleukin-12 subunit alpha; Interleukin12; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; NKSF1