Invitrogen

Human CYB5B (aa 19-112) Control Fragment Recombinant Protein

Name: Human CYB5B (aa 19-112) Control Fragment Recombinant Protein
Brand: Invitrogen
SKU: RP-89712

Datasheet

Tech Support

Datasheet

Tech Support

Product Details

RP-89712

Applications

Tested Dilution

Publications

Control (Ctrl)

Assay-dependent

Blocking Assay (BLOCK)

Assay-dependent

Product Specifications

Species

Human

Expression System

E. coli

Amino acid sequence

EVETSVTYYRLEEVAKRNSLKELWLVIHGRVYDVTRFLNEHPGGEEVLLEQAGVDASESFEDVGHSSDAREMLKQYYIGDIHPSDLKPESGSKD

Tag

His-ABP-tag

Class

Recombinant

Type

Protein

Purity

>80% by SDS-PAGE and Coomassie blue staining

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

≥5.0 mg/mL

Purification

purified

Storage buffer

1M urea/PBS, pH 7.4

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Product Specific Information

Highest antigen sequence indentity to the following orthologs: Mouse (82%), Rat (82%).

This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-52482. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

Target Information

CYB5B gene ontology annotations related to this gene include enzyme activator activity; heme binding; metal ion binding.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Cytochrome b5 outer mitochondrial membrane isoform; Cytochrome b5 type B; cytochrome b5 type B (outer mitochondrial membrane); outer mitochondrial membrane cytochrome b5; type 2 cyt-b5