Invitrogen

Human CWF19L1 (aa 131-223) Control Fragment Recombinant Protein

Name: Human CWF19L1 (aa 131-223) Control Fragment Recombinant Protein
Brand: Invitrogen
SKU: RP-97026

View all (2) CWF19L1 proteins

Datasheet

Tech Support

Datasheet

Tech Support

Product Details

RP-97026

Applications

Tested Dilution

Publications

Control (Ctrl)

Assay-dependent

Blocking Assay (BLOCK)

Assay-dependent

Product Specifications

Species

Human

Expression System

E. coli

Amino acid sequence

KDVSSLRMMLCTTSQFKGVDILLTSPWPKCVGNFGNSSGEVDTKKCGSALVSSLATGLKPRYHFAALEKTYYERLPYRNHIILQENAQHATRF

Tag

His-ABP-tag

Class

Recombinant

Type

Protein

Purity

>80% by SDS-PAGE and Coomassie blue staining

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

≥5.0 mg/mL

Purification

purified

Storage buffer

1M urea/PBS, pH 7.4

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Product Specific Information

Highest antigen sequence indentity to the following orthologs: Mouse (86%), Rat (86%).

This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-57859. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

Target Information

CWF19L1 (CWF19 Like Cell Cycle Control Factor 1) is a gene located on chromosome 10 and encodes a protein involved in the regulation of mRNA splicing and cell cycle control. The protein interacts with key splicing factors within the nucleus, including components of the U5 small nuclear ribonucleoprotein (snRNP) and the pre-mRNA processing factor 19 (PRPF19) complex. CWF19L1 plays a crucial role in the alternative splicing of immune-related genes, thereby influencing the expression levels of cytotoxic molecules and enhancing T-cell cytotoxicity. Pathogenic variants in CWF19L1 have been linked to a rare autosomal recessive form of hereditary ataxia known as SCAR17 (Spinocerebellar Ataxia, Autosomal Recessive 17), characterized by symptoms such as global developmental delay, cerebellar ataxia, pyramidal signs, and seizures. This gene's deficiency disrupts normal mRNA splicing and leads to significant alterations in gene expression, impacting cellular and neurological functions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: C19L1; CWF19-like 1 cell cycle control; CWF19-like protein 1