Invitrogen

Human CLCNKA (aa 525-559) Control Fragment Recombinant Protein

Name: Human CLCNKA (aa 525-559) Control Fragment Recombinant Protein
Brand: Invitrogen
SKU: RP-104514

Datasheet

Tech Support

Datasheet

Tech Support

Product Details

RP-104514

Applications

Tested Dilution

Publications

Control (Ctrl)

Assay-dependent

Blocking Assay (BLOCK)

Assay-dependent

Product Specifications

Species

Human

Expression System

E. coli

Amino acid sequence

IVKKLPYLPRILGRNIGSHHVRVEHFMNHSITTLA

Tag

His-ABP-tag

Class

Recombinant

Type

Protein

Purity

>80% by SDS-PAGE and Coomassie blue staining

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

≥5.0 mg/mL

Purification

purified

Storage buffer

PBS/1M urea, pH 7.4

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Product Specific Information

Highest antigen sequence indentity to the following orthologs: Mouse (74%), Rat (74%).

This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-63472. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

Target Information

CLC-KA is a kidney-specific chloride channel that mediates transepithelial chloride transport in the thin ascending limb of the Henle loop in the inner medulla. CLC-KA plays a crucial role in urine concentration. The gene encoding human CLC-KA maps to chromosome 1p36. Mutations in this gene may be associated with nephrogenic diabetes insipidus in those cases where mutations in the vasopressin V2 receptor and the AQP2 water channel are lacking. CLC-KB mediates basolateral chloride ion efflux in the thick ascending limb and in more distal nephron segments. The gene encoding human CLC-KB maps to chromosome 1p36. Mutations in this gene cause type III Barter's syndrome which is characterized by renal salt-wasting and low blood pressure.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Chloride channel Ka; Chloride channel protein ClC-Ka; chloride channel, kidney, A; chloride channel, voltage-sensitive Ka; ClC-K1; CLC-KA; CLCKA