Invitrogen

Human CCDC115 (aa 96-177) Control Fragment Recombinant Protein

Name: Human CCDC115 (aa 96-177) Control Fragment Recombinant Protein
Brand: Invitrogen
SKU: RP-96090

Datasheet

Tech Support

Datasheet

Tech Support

Product Details

RP-96090

Applications

Tested Dilution

Publications

Control (Ctrl)

Assay-dependent

Blocking Assay (BLOCK)

Assay-dependent

Product Specifications

Species

Human

Expression System

E. coli

Amino acid sequence

PREAGLRRRKGPTKTPEPESSEAPQDPLNWFGILVPHSLRQAQASFRDGLQLAADIASLQNRIDWGRSQLRGLQEKLKQLEP

Tag

His-ABP-tag

Class

Recombinant

Type

Protein

Purity

>80% by SDS-PAGE and Coomassie blue staining

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

≥5.0 mg/mL

Purification

purified

Storage buffer

1M urea/PBS, pH 7.4

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Product Specific Information

Highest antigen sequence indentity to the following orthologs: Mouse (83%), Rat (83%).

This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-56987. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

Target Information

The CCDC115 gene encodes a protein known as coiled-coil domain-containing protein 115, which is involved in cellular processes related to membrane trafficking and Golgi apparatus function. CCDC115 is integral to maintaining Golgi homeostasis and is implicated in the functioning of the vacuolar H+-ATPase (V-ATPase) proton pump, critical for acidifying intracellular compartments. Mutations in CCDC115 have been associated with a genetic condition known as congenital disorder of glycosylation type IIo (CDG-IIo), which leads to defects in protein glycosylation. This disorder results in a spectrum of symptoms, including developmental delays, neurological impairments, and multi-systemic dysfunction due to improper glycosylation of proteins, impacting their stability and function. Research indicates that CCDC115's activity in ion transport and vacuolar acidification is essential for Golgi apparatus function and overall cellular homeostasis. Investigations into CCDC115 are crucial for understanding its role in glycosylation-related disorders and for developing potential therapeutic strategies to manage these conditions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Coiled-coil domain-containing protein 115; Vacuolar ATPase assembly protein VMA22