Invitrogen

Human CCBE1 (aa 159-250) Control Fragment Recombinant Protein

Name: Human CCBE1 (aa 159-250) Control Fragment Recombinant Protein
Brand: Invitrogen
SKU: RP-98285

Datasheet

Tech Support

Datasheet

Tech Support

Product Details

RP-98285

Applications

Tested Dilution

Publications

Control (Ctrl)

Assay-dependent

Blocking Assay (BLOCK)

Assay-dependent

Product Specifications

Species

Human

Expression System

E. coli

Amino acid sequence

CECREGYIREDDGKTCTRGDKYPNDTGHEKSENMVKAGTCCATCKEFYQMKQTVLQLKQKIALLPNNAADLGKYITGDKVLASNTYLPGPPG

Tag

His-ABP-tag

Class

Recombinant

Type

Protein

Purity

>80% by SDS-PAGE and Coomassie blue staining

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

≥5.0 mg/mL

Purification

purified

Storage buffer

PBS/1M urea, pH 7.4

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Product Specific Information

Highest antigen sequence indentity to the following orthologs: Mouse (89%), Rat (89%).

This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-59534. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

Target Information

The gene encoding CCBE1 (Collagen and calcium-binding EGF domain-containing protein 1) is located on chromosome 18 in a region that is frequently found to be deleted in breast and prostate cancers. CCBE1 is a 406 amino acid secreted protein that contains two collagen-like domains and one calcium-binding epidermal growth factor (EGF-like) domain. In general, EGF-like domains include six cysteine residues that are usually involved in disulfide bonds. Calcium-binding EGF-like domains are subject to amino acid substitutions that disrupt their structure or calcium affinity, therefore altering protein function. Downregulation of the gene encoding CCBE1 is observed with high frequency in breast cancer, suggesting that loss of CCBE1 results in changes in cell adhesion and mobility due to its characterization as an extracellular protein. There are three named isoforms of CCBE1 which are produced as a result of alternative splicing events.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Collagen and calcium-binding EGF domain-containing protein 1; FLJ30681; Full of fluid protein homolog; MGC50861