Invitrogen

Human Bcl-B (aa 1-33) Control Fragment Recombinant Protein

Name: Human Bcl-B (aa 1-33) Control Fragment Recombinant Protein
Brand: Invitrogen
SKU: RP-98457
Availability: InStock

View all (2) Bcl-B proteins

Datasheet

Tech Support

Datasheet

Tech Support

Product Details

RP-98457

Applications

Tested Dilution

Publications

Control (Ctrl)

Assay-dependent

Blocking Assay (BLOCK)

Assay-dependent

Product Specifications

Species

Human

Expression System

E. coli

Amino acid sequence

MVDQLRERTTMADPLRERTELLLADYLGYCARE

Tag

His-ABP-tag

Class

Recombinant

Type

Protein

Purity

>80% by SDS-PAGE and Coomassie blue staining

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

≥5.0 mg/mL

Purification

purified

Storage buffer

1M urea/PBS, pH 7.4

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Product Specific Information

Highest antigen sequence indentity to the following orthologs: Mouse (42%), Rat (42%).

This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-111149. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

Target Information

The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The protein encoded by this gene contains conserved BH4, BH1 and BH2 domains. This protein can interact with other members of BCL-2 protein family including BCL2, BCL2L1/BCL-X(L), and BAX. Overexpression of this gene has been shown to suppress cell apoptosis possibly through the prevention of cytochrome C release from the mitochondria, and thus activating caspase-3 activation. The mouse counterpart of this protein is found to interact with Apaf1 and forms a protein complex with Caspase 9, which suggests the involvement of this protein in APAF1 and CASPASE 9 related apoptotic pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Anti-apoptotic protein Boo; Anti-apoptotic protein NrH; Apoptosis regulator Bcl-B; Bcl-2-like protein 10; Bcl2-L-10; BCL2-like 10 (apoptosis facilitator); death inducer binding to vBcl-2 and Apaf-1