Invitrogen

Human BACE1 (beta-Secretase) Recombinant Protein

Name: Human BACE1 (beta-Secretase) Recombinant Protein
Brand: Invitrogen
SKU: P2947

View all (3) BACE1 proteins

Datasheet

Tech Support

Datasheet

Tech Support

Product Details

P2947

Applications

Tested Dilution

Publications

Kinase Assay (KA)

Assay-dependent

Product Specifications

Species

Human

Expression System

Insect cells

Amino acid sequence

TQHGIRLPLR

Molecular weight

55 kDa

Class

Recombinant

Type

Protein

Purity

85% as determined by a Coomassie® blue stained SDS-PAGE gel.

Activity

609 Units/mg. One unit is defined as the amount of BACE1 required to cleave one pmol of Rhodamine-EVNLDAEFK-Quencher in one minute at room temperature. Cleavage of the FRET peptide is monitored by an increase in fluorescence (Ex535 nm/ Em590 nm) in the following assay reaction: 250 nM Rhodamine-EVNLDAEFK-Quencher in 50 mM Sodium Acetate (pH 4.5) in a total volume of 30 µL accoding to the BACE1 (βSecretase) FRET Assay Kit, Red (P2985).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

purified

Storage buffer

proprietary buffer

Contains

no preservative

Storage conditions

-80°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Dry ice

Product Specific Information

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 µg, 10 µg and 20 µg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 µL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase.

Target Information

BACE1 (beta-secretase) is a key enzyme involved in the production of amyloid beta-peptides (Abeta) found in extracellular amyloid plaques of Alzheimer's disease (AD). In some cases early onset familial AD can be attributed to a "Swedish" mutation in the amyloid precursor protein (APP), which dramatically enhances the cleavage of this protein by BACE1. This and other genetic and pathological evidence has led to therapeutic approaches that have focused on the inhibition of BACE1 and other APP-cleaving enzymes, such as gamma-secretase stuff. APP is first cleaved by b-secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for g-secretase to generate the 4 kDa amyloid-b peptide, which is deposited in the brains of all sufferers of Alzheimer's disease. The long-sought b-secretase was recently identified by several groups independently and designated beta-site APP cleaving enzyme (BACE) and aspartyl protease 2 (Asp2). bACE/Asp2 is a novel transmembrane aspartic protease and colocalizes with APP.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: APP beta-secretase; asp 2; ASP2; Aspartyl protease 2; BACE 1; BACE-1D; BACE-I-432; Beta secretase; Beta-secretase 1; beta-secretase 1 precursor variant 1; beta-site amyloid beta A4 precursor protein-cleaving enzyme; Beta-site amyloid precursor protein cleaving enzyme 1; Beta-site APP cleaving enzyme 1; beta-site APP-cleaving enzyme 1; Memapsin; Memapsin-2; Memapsin2; Membrane-associated aspartic protease 2; transmembrane aspartic proteinase Asp2