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  • ATP5J2 Proteins

Invitrogen

Human ATP5J2 (aa 75-94) Control Fragment Recombinant Protein

View all (2) ATP5J2 proteins

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Datasheet
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Datasheet
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Cite Human ATP5J2 (aa 75-94) Control Fragment Recombinant Protein

Product Details

RP-105503

Applications
Tested Dilution
Publications

Control (Ctrl)

Assay-dependent
-

Blocking Assay (BLOCK)

Assay-dependent
-
Product Specifications

Species

Human

Expression System

E. coli

Amino acid sequence

LFSYSFSYKHLKHERLRKYH

Tag

His-ABP-tag

Class

Recombinant

Type

Protein

Purity

>80% by SDS-PAGE and Coomassie blue staining

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

≥5.0 mg/mL

Purification

purified

Storage buffer

PBS/1M urea, pH 7.4

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Product Specific Information

Highest antigen sequence indentity to the following orthologs: Mouse (75%), Rat (75%).

This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-67104. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

Target Information

ATP5J2 is part of the complex F0 domain of the mitochondrial ATP synthase. This produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. It is composed of two complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. ATP5J2 encodes the f subunit of the Fo complex. There are 2 isoforms produced by alternative splicing.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ATP synthase f chain, mitochondrial; ATP synthase F(0) complex subunit f, mitochondrial; ATP synthase membrane subunit f; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit f, isoform 2; ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F2; F1F0-type ATPase subunit f; F1Fo-ATP synthase complex Fo membrane domain f subunit; F1Fo-ATPase synthase f subunit

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Gene Aliases: ATP5J2; ATP5JL; ATP5MF

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UniProt ID: (Human) P56134

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Entrez Gene ID: (Human) 9551

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It has to be done as per old AB suggested Products section.
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