Invitrogen

Human ATP4A (aa 878-915) Control Fragment Recombinant Protein

Name: Human ATP4A (aa 878-915) Control Fragment Recombinant Protein
Brand: Invitrogen
SKU: RP-95785

View all (2) ATP4A proteins

Datasheet

Tech Support

Datasheet

Tech Support

Product Details

RP-95785

Applications

Tested Dilution

Publications

Control (Ctrl)

Assay-dependent

Blocking Assay (BLOCK)

Assay-dependent

Product Specifications

Species

Human

Expression System

E. coli

Amino acid sequence

TDYFTAMAQEGWFPLLCVGLRAQWEDHHLQDLQDSYGQ

Tag

His-ABP-tag

Class

Recombinant

Type

Protein

Purity

>80% by SDS-PAGE and Coomassie blue staining

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

≥5.0 mg/mL

Purification

purified

Storage buffer

PBS/1M urea, pH 7.4

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Product Specific Information

Highest antigen sequence indentity to the following orthologs: Mouse (95%), Rat (95%).

This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-58673. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

Target Information

The ATP4A gene encodes the alpha subunit of the gastric H+/K+ ATPase, an enzyme crucial for gastric acid secretion in the stomach. This proton pump resides in the parietal cells of the gastric mucosa and is responsible for exchanging potassium ions (K+) for hydrogen ions (H+), effectively acidifying the stomach contents. ATP4A plays a significant role in maintaining the acidic environment necessary for digestion and defense against pathogens. The ATPase's activity is tightly regulated by various factors, including hormones like gastrin and histamine, to ensure optimal stomach function. Mutations in ATP4A can lead to disorders related to gastric acidity, such as hypochlorhydria (low stomach acid), which can impact digestion and absorption of nutrients. Understanding ATP4A's function and its regulatory mechanisms is essential for developing treatments for conditions like peptic ulcer disease and gastroesophageal reflux disease, where gastric acid secretion is disrupted.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: ATPase, H+/K+ exchanging, alpha polypeptide; ATPase, H+/K+ transporting, alpha polypeptide; Gastric H(+)/K(+) ATPase subunit alpha; gastric H+/K+ ATPase alpha subunit; gastric H,K-ATPase alpha subunit; gastric H,K-ATPase catalytic subunit; gastric hydrogen-potassium ATPase; H(+)-K(+)-ATPase alpha subunit; Potassium-transporting ATPase alpha chain 1; Proton pump