Invitrogen

Human ARAP2 (aa 1614-1702) Control Fragment Recombinant Protein

Name: Human ARAP2 (aa 1614-1702) Control Fragment Recombinant Protein
Brand: Invitrogen
SKU: RP-96597

Datasheet

Tech Support

Datasheet

Tech Support

Product Details

RP-96597

Applications

Tested Dilution

Publications

Control (Ctrl)

Assay-dependent

Blocking Assay (BLOCK)

Assay-dependent

Product Specifications

Species

Human

Expression System

E. coli

Amino acid sequence

HCLEHKDDKLRNRPRKHRSFNCLEDTEPEAPLGQPKGHKGLKTLRKTEDRNSKATLDSDHKLPSRVIEELNVVLQRSRTLPKELQDEQI

Tag

His-ABP-tag

Class

Recombinant

Type

Protein

Purity

>80% by SDS-PAGE and Coomassie blue staining

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

≥5.0 mg/mL

Purification

purified

Storage buffer

PBS/1M urea, pH 7.4

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Product Specific Information

Highest antigen sequence indentity to the following orthologs: Mouse (79%), Rat (79%).

This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-57465. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

Target Information

ARAP2 also known as Centaurin-delta-1 is a protein with ARF-GAP, RHO-GAP, ankyrin repeat, RAS-associating, and pleckstrin homology domains. A phosphatidylinositol-3, 4, 5-trisphosphate-dependent GTPase-activating protein, ARAP2, binds to RhoA-GTP, but lacks the predicted catalytic arginine in the RHO-GAP domain and does not have RHO-GAP activity. It modulates actin cytoskeleton remodeling by regulating ARF and RHO family members. Usually ARAP2 is activated by phosphatidylinositol-3, 4, 5-trisphosphate (PtdIns(3, 4, 5)P3) binding and can be activated by phosphatidylinositol-3, 4-bisphosphate (PtdIns(3, 4, 5)P2) binding, albeit with lower efficiency. ARAP2 associates with focal adhesions and functions downstream of RhoA to regulate focal adhesion dynamics. It is usually detected in brain, thymus, lymph node, thyroid, spinal cord, trachea, heart, skeletal muscle, spleen, kidney, liver, placenta, lung and peripheral blood leukocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Arf and Rho GAP adapter protein 2; Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 2; centaurin; centaurin, delta 1; Centaurin-delta-1; Cnt-d1; Protein PARX