Invitrogen

Human AP1S2 (aa 178-202) Control Fragment Recombinant Protein

Name: Human AP1S2 (aa 178-202) Control Fragment Recombinant Protein
Brand: Invitrogen
SKU: RP-100849

Datasheet

Tech Support

Datasheet

Tech Support

Product Details

RP-100849

Applications

Tested Dilution

Publications

Control (Ctrl)

Assay-dependent

Blocking Assay (BLOCK)

Assay-dependent

Product Specifications

Species

Human

Expression System

E. coli

Amino acid sequence

QADLLQEKTETMYHSKSFIGFKKAY

Tag

His-ABP-tag

Class

Recombinant

Type

Protein

Purity

>80% by SDS-PAGE and Coomassie blue staining

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

≥5.0 mg/mL

Purification

purified

Storage buffer

PBS/1M urea, pH 7.4

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Product Specific Information

Highest antigen sequence indentity to the following orthologs: Mouse (96%), Rat (96%).

This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-62081. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

Target Information

Adaptor protein complex 1 is found at the cytoplasmic face of coated vesicles located at the Golgi complex, where it mediates both the recruitment of clathrin to the membrane and the recognition of sorting signals within the cytosolic tails of transmembrane receptors. This complex is a heterotetramer composed of two large, one medium, and one small adaptin subunit. The protein encoded by this gene serves as the small subunit of this complex and is a member of the adaptin protein family. Transcript variants utilizing alternative polyadenylation signals exist for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: adapter-related protein complex 1 sigma-1B subunit; adaptor protein complex AP-1 sigma-1B subunit; Adaptor protein complex AP-1 subunit sigma-1B; adaptor-related protein complex 1 sigma 2 subunit; Adaptor-related protein complex 1 subunit sigma-1B; adaptor-related protein complex 1, sigma 2 subunit; AP-1 complex subunit sigma-2; clathrin adaptor complex AP1 sigma 1B subunit; Clathrin assembly protein complex 1 sigma-1B small chain; Golgi adaptor HA1/AP1 adaptin sigma-1B subunit; MGC:1902; Sigma 1B subunit of AP-1 clathrin; Sigma-adaptin 1B; Sigma1B-adaptin