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  • AMPK alpha-2/beta-2/gamma-2 Proteins

Invitrogen

Human AMPK alpha-2/beta-2/gamma-2, His Tag Recombinant Protein

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Datasheet
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Datasheet
Tech Support

Cite Human AMPK alpha-2/beta-2/gamma-2, His Tag Recombinant Protein

Product Details

PV6250

Applications
Tested Dilution
Publications

Kinase Assay (KA)

Assay-dependent
-
Product Specifications

Species

Human

Expression System

Baculovirus

Amino acid sequence

1-552, 1-272, 1-569

Tag

His-tag

Molecular weight

63.1-36-41 kDa

Class

Recombinant

Type

Protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

purified

Storage buffer

50mM sodium phosphate, pH 7, with 300mM NaCl, 0.1mM PMSF, 0.2mM DTT, 150mM Imidazole, 25% glycerol

Contains

no preservative

Storage conditions

-80°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Dry ice

Product Specific Information

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 µg, 10 µg and 20 µg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 µL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase.

Recombinant full-length human AMPK (combination of A2/B2/G2 subunits) was expressed by baculovirus in Sf9 insect cells using C-terminal His tag.

• Validated in LanthaScreen™ Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use

Related Product:
LanthaScreen™ Eu Kinase Binding Assay

Protein Form: Recombinant

Target Information

AMPK (A2/B2/G2) is a member of the AMPK family which are heterotrimeric proteins consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPKs are an important energy-sensing enzyme group in the cells that monitor energy status particularly in response to stress. AMPKs regulate fatty acid and cholesterol synthesis by regulating the key rate-limiting enzymes acetyl-CoA carboxylase and hydroxy beta-methylglutaryl-CoA reductase. The beta subunit may be a positive regulator of AMPK activity and is highly expressed in skeletal muscle.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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