Invitrogen

Human ALKBH7 (aa 21-92) Control Fragment Recombinant Protein

Name: Human ALKBH7 (aa 21-92) Control Fragment Recombinant Protein
Brand: Invitrogen
SKU: RP-102874

View all (3) ALKBH7 proteins

Datasheet

Tech Support

Datasheet

Tech Support

Product Details

RP-102874

Applications

Tested Dilution

Publications

Control (Ctrl)

Assay-dependent

Blocking Assay (BLOCK)

Assay-dependent

Product Specifications

Species

Human

Expression System

E. coli

Amino acid sequence

SGPSVLSRLQDAAVVRPGFLSTAEEETLSRELEPELRRRRYEYDHWDAAIHGFRETEKSRWSEASRAILQRV

Tag

His-ABP-tag

Class

Recombinant

Type

Protein

Purity

>80% by SDS-PAGE and Coomassie blue staining

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

≥5.0 mg/mL

Purification

purified

Storage buffer

PBS/1M urea, pH 7.4

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Product Specific Information

Highest antigen sequence indentity to the following orthologs: Mouse (78%), Rat (78%).

This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-58842. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

Target Information

ALKBH7 (alkB, alkylation repair homolog 7), also known as SPATA11, is a 221 amino acid protein belonging to the alkB family. ALKBH7 is one of many homologs of the Escherichia coli protein AlkB. AlkB functions to protect DNA and RNA against damage from environmental methylating compounds by directly reversing 1-methyladenine (1-meA) and 3-methylcytosine (3-meC) cytotoxic alkylation lesions in DNA and RNA. The enzyme acts by oxidative demethylation, utilizing ferrous iron and alpha-ketoglutarate as cofactors, 2-oxoglutarate as a co-substrate and molecular oxygen as the oxidizing agent. ALKBH7 is encoded by a gene located on human chromosome 19, which consists of over 63 million bases, houses approximately 1,400 genes and is recognizedfor having the greatest gene density of the human chromosomes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: alkB, alkylation repair homolog 7; Alkylated DNA repair protein alkB homolog 7; Alpha-ketoglutarate-dependent dioxygenase alkB homolog 7, mitochondrial; probable alpha-ketoglutarate-dependent dioxygenase ABH7; spermatogenesis associated 11; spermatogenesis cell proliferation related protein; Spermatogenesis cell proliferation-related protein; Spermatogenesis-associated protein 11