Invitrogen

Human AKT1 (PKB alpha), His Tag Recombinant Protein

Name: Human AKT1 (PKB alpha), His Tag Recombinant Protein
Brand: Invitrogen
SKU: P2999

Datasheet

Tech Support

Datasheet

Tech Support

Product Details

P2999

Applications

Tested Dilution

Publications

Kinase Assay (KA)

Assay-dependent

Product Specifications

Species

Human

Expression System

Insect cells

Amino acid sequence

Full length

Tag

His-tag

Molecular weight

59.1 kDa

Class

Recombinant

Type

Protein

Purity

90% by SDS-PAGE

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

purified

Storage buffer

20mM tris, pH 7.5, with 0.1mM sodium orthovanadate, 20% glycerol, 2mM DTT, 0.01% Triton X-100, 150mM NaCl, 0.5mM EDTA, 0.1mM sodium orthovanadate

Contains

no preservative

Storage conditions

-80°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Dry ice

Product Specific Information

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 µg, 10 µg and 20 µg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 µL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase.

Protein Form: Full Length

Target Information

AKT1 (PKB alpha) is a serine/threonine kinase that regulates cell survival. The activated enzyme inhibits apoptosis and stimulates cell cycle progression by phosphorylating numerous targets in various cell types, including cancer cells. This protein kinase is activated by insulin, PI3K, IGF1 and various other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. The AKT pathway is a major target for cancer drug discovery.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: AKT1m; C-AKT; CAKT; PKB; PKB alpha; PKB beta; PKB gamma; PKBG; Protein kinase B; Protein kinase B alpha; Proto-oncogene c-Akt; rac protein kinase alpha; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha; RAC-PK-beta; RAC-PK-gamma; serine-threonine protein kinase; v-akt murine thymoma viral oncogene homolog 1; v-akt murine thymoma viral oncogene-like protein 1