Invitrogen

Human ADPGK (aa 99-178) Control Fragment Recombinant Protein

Name: Human ADPGK (aa 99-178) Control Fragment Recombinant Protein
Brand: Invitrogen
SKU: RP-106863
Availability: InStock

View all (2) ADPGK proteins

Datasheet

Tech Support

Datasheet

Tech Support

Product Details

RP-106863

Applications

Tested Dilution

Publications

Control (Ctrl)

Assay-dependent

Blocking Assay (BLOCK)

Assay-dependent

Product Specifications

Species

Human

Expression System

E. coli

Amino acid sequence

SPGNGKDHSILHSRNDLEEAFIHFMGKGAAAERFFSDKETFHDIAQVASEFPGAQHYVGGNAALIGQKFAANSDLKVLLC

Tag

His-ABP-tag

Class

Recombinant

Type

Protein

Purity

>80% by SDS-PAGE and Coomassie blue staining

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

≥5.0 mg/mL

Purification

purified

Storage buffer

1M urea/PBS, pH 7.4

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Product Specific Information

Highest antigen sequence indentity to the following orthologs: Mouse (90%), Rat (90%).

This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-66176. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

Target Information

The ADPGK gene encodes ADP-dependent glucokinase, an enzyme distinct from the ATP-dependent hexokinases as it employs adenosine diphosphate (ADP) rather than ATP to phosphorylate glucose to glucose-6-phosphate. This gene, initially identified in archaeal species, has been co-opted into mammalian systems where it plays roles primarily in glycolytic processes. Structurally, the mammalian ADPGK differs from its archaeal counterpart, exemplified by the rearrangement of its secondary structures, such as the alpha2 and alpha3 helices. These helices are replaced by an extended beta-strand region, indicating evolutionary adaptations for function in higher organisms like mice. Although its precise physiological role remains partially defined, ADPGK is notably expressed in both hematopoietic lineages and tumor tissues, suggesting potential significance in cancer biology and energy metabolism.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ADP-dependent glucokinase; ADP-GK; ATP-dependent glucokinase; EC 2.7.1.147; RbBP-35