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  • Primary Antibodies ›
  • beta Actin Antibodies

Invitrogen

beta Actin Monoclonal Antibody (BA3R), HRP

33 Published Figures
44 References
View all (92) beta Actin antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite beta Actin Monoclonal Antibody (BA3R), HRP

  • Antibody Testing Data (2)
  • Published Figures (33)
beta Actin Antibody in Western Blot (WB)
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beta Actin Antibody in Western Blot (WB)
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beta Actin Antibody (MA5-15739-HRP) in WB

Western blot analysis was performed on whole cell extracts (30 µg lysate) of A549 (Lane 1), COS-7 (Lane 2), MDCK (Lane 3), C2C12 (Lane 4), MDA-MB-231 (Lane 5), RSC96 (Lane 6) and tissue extracts of Mouse Lung (Lane 7). The blot was probed with beta Actin Monoclonal Antibody (BA3R), HRP (Product # MA5-15739-HRP, 1 µg/mL) and detected by chemiluminescence. A 42 kDa band corresponding to beta Actin was observed across the cell lines and tissues tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10% Bis-Tris ge... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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beta Actin Antibody in Western Blot (WB)
beta Actin Antibody in Western Blot (WB)
beta Actin Antibody in Western Blot (WB)
beta Actin Antibody in Western Blot (WB)
beta Actin Antibody in Western Blot (WB)
beta Actin Antibody in Western Blot (WB)
beta Actin Antibody in Western Blot (WB)
beta Actin Antibody in Western Blot (WB)
beta Actin Antibody in Western Blot (WB)
beta Actin Antibody in Western Blot (WB)
beta Actin Antibody in Western Blot (WB)
beta Actin Antibody in Western Blot (WB)
beta Actin Antibody in Western Blot (WB)
beta Actin Antibody in Western Blot (WB)
beta Actin Antibody in Western Blot (WB)
beta Actin Antibody in Western Blot (WB)
beta Actin Antibody in Western Blot (WB)
beta Actin Antibody in Western Blot (WB)
beta Actin Antibody in Western Blot (WB)
beta Actin Antibody in Western Blot (WB)
beta Actin Antibody in Western Blot (WB)
beta Actin Antibody in Western Blot (WB)
beta Actin Antibody in Western Blot (WB)
beta Actin Antibody in Western Blot (WB)
beta Actin Antibody in Western Blot (WB)
beta Actin Antibody in Western Blot (WB)
beta Actin Antibody in Western Blot (WB)
beta Actin Antibody in Western Blot (WB)
beta Actin Antibody in Western Blot (WB)
beta Actin Antibody in Western Blot (WB)
beta Actin Antibody in Western Blot (WB)
beta Actin Antibody in Western Blot (WB)
beta Actin Antibody in Western Blot (WB)
beta Actin Antibody in Western Blot (WB)
beta Actin Antibody in Western Blot (WB)
beta Actin Monoclonal Antibody (BA3R), HRP

Product Details

MA5-15739-HRP

Applications
Tested Dilution
Publications

Western Blot (WB)

1 µg/mL
View 43 publications 43 publications

Immunocytochemistry (ICC/IF)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Dog, Chicken, Human, Mouse, Non-human primate, Rabbit, Rat

Published species

Fruit fly, Hamster, Human, Mouse, Rat

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

BA3R

Immunogen

Beta-actin N-terminal peptide.
View immunogen

Conjugate

HRP HRP HRP
  • Unconjugated
  • Biotin 
  • DyLight 488
  • DyLight 550
  • DyLight 650
  • DyLight 680
  • DyLight 800 4X PEG
  • Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.07% Kathon

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2537667

Product Specific Information

MA5-15739-HRP has been successfully used in Western blotting applications with human, mouse, rat, rabbit, and chicken samples.

Target Information

Beta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: actin, alpha 1, skeletal muscle; actin, beta; Actin, cytoplasmic 1; beta cytoskeletal actin; Beta-actin; cytoplasmic 1; cytoplasmic beta-actin; gamma non-muscle actin; PS1TP5-binding protein 1

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Gene Aliases: ACT-5; ACTA1; ACTB; actin; Actx; Bact; beta-actin; BRWS1; E430023M04Rik; PS1TP5BP1; RCJMB04_4h19

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UniProt ID: (Rabbit) P29751, (Human) P60709, (Chicken) P60706, (Dog) O18840, (Rat) P60711, (Mouse) P60710

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Entrez Gene ID: (Rabbit) 100009272, (Human) 60, (Chicken) 396526, (Dog) 403580, (Rat) 81822, (Mouse) 11461

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding structural constituent of cytoskeleton protein binding ATP binding kinesin binding Tat protein binding nucleosomal DNA binding identical protein binding nitric-oxide synthase binding protein kinase binding
Process(es)
retina homeostasis movement of cell or subcellular component cytoskeleton organization substantia nigra development cell junction assembly Fc-gamma receptor signaling pathway involved in phagocytosis ATP-dependent chromatin remodeling positive regulation of gene expression, epigenetic vascular endothelial growth factor receptor signaling pathway ephrin receptor signaling pathway platelet aggregation axonogenesis retina development in camera-type eye cellular response to electrical stimulus
It has to be done as per old AB suggested Products section.
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