 |  |  |  |  |  |  |
| 4 | 405 | 450/50 | 413 | 431 | (in buffer) 5 | flow cytometry |
Invitrogen eBioscience Super Bright 436 dye is a polymer-based fluorophore excited by the violet laser (405 nm) that emits fluorescence signal at 436 nm with high efficacy. The technology behind the Super Bright polymer dye family makes them useful for identifying low-abundance markers on a cell surface in flow cytometry because they emit fluorescence with high efficacy. Super Bright 436 antibody conjugates are an alternative to Brilliant Violet 421 conjugates, offering increased resolution of positive and negative populations with less background and less interfering dye-dye interactions. In addition, antibody conjugates of Super Bright 436 are significantly brighter than eFluor 450 conjugates and Pacific Blue conjugates.
Super Bright conjugates are fully compatible with UltraComp eBeads microspheres for compensation, viability stains, fluorescent dyes and other reagents commonly used in flow cytometry. Robust, highly sensitive, and stable, Super Bright conjugates easily fit into any flow cytometry panel. When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, we recommend using Super Bright Complete Staining Buffer or Brilliant Stain Buffer to minimize any non-specific polymer interactions.
We offer Super Bright 436 dye conjugated to primary antibodies and streptavidin for use in flow cytometry.
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Super Bright dyes
Super Bright polymer dyes are violet laser–excitable fluorophores that expand the capabilities of multi-parametric flow cytometry analysis. This family of bright fluorophores enables the use of more markers off the violet channel and increases the detection of low-abundance antigens due to their super bright signals. The Super Bright polymer dyes join the trusted Invitrogen Alexa Fluor dye and Invitrogen eFluor dye portfolios in providing fluorochromes compatible with the 405 nm spectral line of the violet laser.