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Invitrogen

Human STAT5 alpha/beta (Phospho) [pY694/pY699] InstantOne™ ELISA Kit

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Technical guide & protocol Tech Support
Technical guide & protocol Tech Support
Human STAT5 alpha/beta (Phospho) [pY694/pY699] InstantOne™ ELISA Kit
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Human STAT5 alpha/beta (Phospho) [pY694/pY699] InstantOne™ ELISA Kit
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Human STAT5 alpha/beta (Phospho) [pY694/pY699] InstantOne™ ELISA Kit

Representative data for STAT5 alpha/beta (Phospho) [pY694/pY699] Multispecies InstantOne™ ELISA. A431 cells were pre-treated with 10 µM AG1478 (an EGFR kinase inhibitor), washed and unstimulated (-) or stimulated with 10 ng/mL EGF for 10 min (+). Cells were analyzed by immunoblotting (left) and InstantOne™ ELISA (right). The intensity of the band between 85 and 92 kDa as detected by immunoblot correlates wi... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Human STAT5 alpha/beta (Phospho) [pY694/pY699] InstantOne™ ELISA Kit
Human STAT5 alpha/beta (Phospho) [pY694/pY699] InstantOne™ ELISA Kit (85-86112-11)
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Product Specifications

Sample type/volume

Cell Lysate
20 µL

Hands-on time

1 hr 20 min

Time-to-result

1 hr 10 min

Homogenous (no wash)

No

Instrument

Absorbance Microplate Reader

Product size

96 Tests

Contents

Capture Antibody
Detection Antibody Reagent
InstantOne™ Assay Plate
Cell Lysis Buffer
Enhancer Solution
Wash Buffer (10X)
Substrate Reagent (TMB)
Stop Solution
Positive Control Cell Lysate (lyophilized)
Plate seal

Shipping conditions

Wet ice

Storage

2-8°C

Protein name

STAT5 alpha/beta

Target site-specificity

Phosphorylated Tyr694, Phosphorylated Tyr699

Species (tested)

Human

Assay kit format

InstantOne ELISA

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

The InstantOne™ ELISA is specifically engineered for accurate measurement of phosphorylated human STAT5A/B in cell lysates. Detection of mouse and rat STAT5A/B is expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.

Target information

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor-associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of, many cell ligands, such as IL-2, IL-3, IL-7, GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/ BCL-X(L), which suggests the antiapoptotic function of this gene in cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene aliases : MGF, STAT5, STAT5A, STAT5B

Gene ID : (Human) 6776, (Human) 6777

Gene symbol : STAT5A, STAT5B

Protein Aliases : Signal transducer and activator of transcription 5A, Signal transducer and activator of transcription 5B, STA5B, STAT, transcription factor STAT5B

UniProt ID (Human) P42229, (Human) P51692

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SIZE

10 x 96 tests

PRICE

USD 400.00

Cat # CHC1263

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Performance Guarantee

If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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