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Invitrogen

Mouse FLT-3L ELISA Kit

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Technical guide & protocol Tech Support
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Technical guide & protocol Tech Support
Mouse FLT-3L ELISA Kit
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Mouse FLT-3L ELISA Kit
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Mouse FLT-3L ELISA Kit

ELISA Standard curve of FLT-3L using Mouse FLT-3L ELISA Kit (Product # EMFLT3L). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Mouse FLT-3L ELISA Kit
Mouse FLT-3L ELISA Kit (EMFLT3L)
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Product Specifications

Analytical sensitivity

15 pg/mL

Assay range

20.48-5,000 pg/mL

Sample type/volume

Plasma
50 µL
Serum
50 µL
Supernatant
100 µL

Hands-on time

1 hr 20 min

Time-to-result

4 hr 45 min

Homogenous (no wash)

No

Interassay CV

<12%

Intraassay CV

<10%

Instrument

Absorbance Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C

Protein name

FLT3LG

Protein family

Proteases Peptidases & Inhibitors

Species (tested)

Mouse

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

The Mouse Flt-3L ELISA quantitates Ms Flt-3L in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms Flt-3L.

Principle of the method

The Mouse Flt-3L solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Target information

FLT3LG (Fms Related Tyrosine Kinase 3 Ligand) is a Protein Coding gene. Diseases associated with FLT3LG include Aplastic Anemia and Brain Cancer. Among its related pathways are Hematopoietic Stem Cell Differentiation Pathways and Lineage-specific Markers and Ras signaling pathway. Gene Ontology (GO) annotations related to this gene include protein homodimerization activity and cytokine activity. Dendritic cells (DCs) provide the key link between innate and adaptive immunity by recognizing pathogens and priming pathogen-specific immune responses. FLT3LG controls the development of DCs and is particularly important for plasmacytoid DCs and CD8-positive classical DCs and their CD103-positive tissue counterparts.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene aliases : Flt3l, Flt3lg, Ly72L

Gene ID : (Mouse) 14256

Gene symbol : Flt3l

Protein Aliases : Flt3 ligand, Flt3L, SL cytokine, H-FLT-3-L, M-FLT-3-L, Flt 3 ligand, Flt3 L, Fms-related tyrosine kinase 3 ligand

UniProt ID (Mouse) P49772

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SIZE

10 x 96 tests

PRICE

USD 400.00

Cat # CHC1263

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Performance Guarantee

If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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