Osenses

nNOS Polyclonal Antibody

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View all (25) nNOS antibodies

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Datasheet
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Datasheet
Protocols
Questions & Answers
nNOS Polyclonal Antibody

Product Details

OSN00004G-500UG

Applications
Tested Dilution
Publications

Western Blot (WB)

10-50 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100
-

Miscellaneous PubMed (MISC)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Guinea pig, Human, Mouse, Rabbit, Rat

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptides from the c-terminal region of human nNOS conjugated to blue carrier protein was used as the antigen
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

Conc. Not Determined

Purification

Ammonium sulfate precipitation

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.

Specificity of this antibody: nNOS.

Target Information

NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: bNOS; bNOS1; Brain NOS; Constitutive NOS; N-NOS; NC-NOS; Neuronal NOS; nitric oxidase synthase; Nitric oxide synthase 1; nitric oxide synthase 1 (neuronal); Nitric oxide synthase, brain; nNOS; NOS Type 1; NOS type I; Peptidyl-cysteine S-nitrosylase NOS1; Phospho-bNOS; Phospho-Brain NOS

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Gene Aliases: 2310005C01Rik; Bnos; IHPS1; N-NOS; NC-NOS; nNOS; NO; NOS; Nos-1; NOS-I; NOS1

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UniProt ID: (Human) P29475, (Rabbit) O19132, (Rat) P29476, (Mouse) Q9Z0J4

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Entrez Gene ID: (Human) 4842, (Rabbit) 100009243, (Rat) 24598, (Mouse) 18125, (Guinea pig) 100729682

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Function(s)
nitric-oxide synthase activity iron ion binding protein binding calmodulin binding FMN binding sodium channel regulator activity enzyme binding heme binding tetrahydrobiopterin binding arginine binding protein homodimerization activity ion channel binding cadmium ion binding flavin adenine dinucleotide binding NADP binding scaffold protein binding NADPH-hemoprotein reductase activity amino acid binding ATPase binding
Process(es)
response to hypoxia regulation of sodium ion transport arginine catabolic process nitric oxide biosynthetic process muscle contraction striated muscle contraction nitric oxide mediated signal transduction female pregnancy aging regulation of heart contraction negative regulation of cell proliferation response to heat response to organonitrogen compound response to lead ion response to organic cyclic compound response to activity peptidyl-cysteine S-nitrosylation positive regulation of guanylate cyclase activity response to nutrient levels response to lipopolysaccharide negative regulation of peptidyl-serine phosphorylation response to vitamin E multicellular organismal response to stress positive regulation of histone acetylation response to nicotine exogenous drug catabolic process negative regulation of apoptotic process negative regulation of potassium ion transport response to peptide hormone response to estrogen response to ethanol negative regulation of blood pressure negative regulation of heart contraction positive regulation of transcription, DNA-templated negative regulation of vasoconstriction positive regulation of vasodilation positive regulation of transcription from RNA polymerase II promoter negative regulation of insulin secretion behavioral response to cocaine negative regulation of hydrolase activity negative regulation of cytosolic calcium ion concentration negative regulation of serotonin uptake negative regulation of calcium ion transport regulation of sensory perception of pain oxidation-reduction process cellular response to mechanical stimulus cellular response to growth factor stimulus response to nitric oxide cellular response to epinephrine stimulus positive regulation of the force of heart contraction positive regulation of long-term synaptic potentiation positive regulation of adrenergic receptor signaling pathway involved in heart process positive regulation of neuron death positive regulation of sodium ion transmembrane transport myoblast fusion negative regulation of calcium ion transport into cytosol neurotransmitter biosynthetic process cell redox homeostasis regulation of neurogenesis regulation of cardiac muscle contraction regulation of ryanodine-sensitive calcium-release channel activity negative regulation of adrenergic receptor signaling pathway involved in heart process regulation of calcium ion transmembrane transport via high voltage-gated calcium channel regulation of cardiac conduction
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