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  • hnRNP C Antibodies

Invitrogen

hnRNP C Polyclonal Antibody

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1 Reference
View all (14) hnRNP C antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite hnRNP C Polyclonal Antibody

  • Antibody Testing Data (9)
  • Advanced Verification (1)
hnRNP C Antibody in Immunocytochemistry (ICC/IF)
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hnRNP C Antibody in Immunocytochemistry (ICC/IF)
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hnRNP C Antibody (PA5-22280) in ICC/IF

Immunofluorescence analysis of hnRNP C was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with hnRNP C Rabbit Polyclonal Antibody(Product # PA5-22280) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A2... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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hnRNP C Antibody in Immunocytochemistry (ICC/IF)
hnRNP C Antibody in Immunocytochemistry (ICC/IF)
hnRNP C Antibody in Immunohistochemistry (Paraffin) (IHC (P))
hnRNP C Antibody in Immunohistochemistry (Paraffin) (IHC (P))
hnRNP C Antibody in Western Blot (WB)
hnRNP C Antibody in Western Blot (WB)
hnRNP C Antibody in Western Blot (WB)
hnRNP C Antibody in Western Blot (WB)
hnRNP C Antibody in Western Blot (WB)
hnRNP C Antibody

Product Details

PA5-22280

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
- -

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
-

Immunocytochemistry (ICC/IF)

1:100-1:1,000
View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent
- -
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human hnRNP C1/C2. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.79 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 1% BSA, 10% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11156187

Product Specific Information

Recommended positive controls: Raji, Neuro2A, NIH-3T3, BCL-1, Raw264.7, C2C12, PC-12, Rat2.

Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (88%), Pig (100%), Rabbit (92%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene can act as a tetramer and is involved in the assembly of 40S hnRNP particles. Multiple transcript variants encoding at least two different isoforms have been described for this gene.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: heterogeneous nuclear ribonucleoprotein C; heterogeneous nuclear ribonucleoprotein C (C1/C2); heterogeneous nuclear ribonucleoprotein C1; heterogeneous nuclear ribonucleoprotein C2; Heterogeneous nuclear ribonucleoproteins C1/C2; heterogeneous ribonucleoprotein C1/C2; hnRNP C protein C-terminal fragment (158 AA); hnRNP C1 / hnRNP C2; hnRNP C1/C2; hnRNP C2; hnRNP core protein C; nuclear ribonucleoprotein particle C1 protein; nuclear ribonucleoprotein particle C2 protein; snoRNA MBI-122

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Gene Aliases: AL022939; C1; C2; D14Wsu171e; HNRNP; hnRNP C; hnrnp-C; HNRNPC; hnRNPC1; hnRNPC2; HNRPC; Hnrpc1; Hnrpc2; SNRPC

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UniProt ID: (Human) P07910, (Mouse) Q9Z204

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Entrez Gene ID: (Human) 3183, (Rat) 290046, (Mouse) 15381

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Function(s)
nucleotide binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding RNA binding mRNA 3'-UTR binding protein binding poly(U) RNA binding nucleosomal DNA binding identical protein binding poly(A) RNA binding telomerase RNA binding N6-methyladenosine-containing RNA binding nucleic acid binding mRNA binding
Process(es)
osteoblast differentiation mRNA processing RNA splicing ATP-dependent chromatin remodeling 3'-UTR-mediated mRNA stabilization mRNA splicing, via spliceosome gene expression protein sumoylation negative regulation of telomere maintenance via telomerase
It has to be done as per old AB suggested Products section.
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