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  • Primary Antibodies ›
  • eIF4E2 Antibodies

Invitrogen

eIF4E2 Polyclonal Antibody

View all (25) eIF4E2 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite eIF4E2 Polyclonal Antibody

  • Antibody Testing Data (4)
eIF4E2 Antibody in Immunocytochemistry (ICC/IF)
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eIF4E2 Antibody in Immunocytochemistry (ICC/IF)
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FIGURE: 1 / 4

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eIF4E2 Antibody (PA5-86981) in ICC/IF

Immunocytochemistry analysis of eIF4E2 in NIH-3T3 cells. Samples were incubated with eIF4E2 polyclonal antibody (Product # PA5-86981) using a dilution of 1:100. Blue: DAPI for nuclear staining. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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eIF4E2 Antibody in Immunocytochemistry (ICC/IF)
eIF4E2 Antibody in Immunocytochemistry (ICC/IF)
eIF4E2 Antibody in Western Blot (WB)
eIF4E2 Antibody in Western Blot (WB)

Product Details

PA5-86981

Applications
Tested Dilution
Publications

Western Blot (WB)

1:200-1:2,000
-

Immunohistochemistry (IHC)

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein of human EIF4E2.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2803737

Product Specific Information

Purity is > 95% by SDS-PAGE.

Target Information

Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation (PubMed:9582349, PubMed:17368478, PubMed:25624349). Acts as a repressor of translation initiation (PubMed:22751931). In contrast to EIF4E, it is unable to bind eIF4G (EIF4G1, EIF4G2 or EIF4G3), suggesting that it acts by competing with EIF4E and block assembly of eIF4F at the cap. [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: eIF-4E type 2; eIF4E type 2; eIF4E-2; eIF4E-like cap-binding protein; eIF4E-like protein 4E-LP; eukar; Eukaryotic translation initiation factor 4E homologous protein; eukaryotic translation initiation factor 4E like 3; eukaryotic translation initiation factor 4E member 2; Eukaryotic translation initiation factor 4E type 2; Eukaryotic translation initiation factor 4E-like 3; h4EHP; mRNA cap-binding protein 4EHP; mRNA cap-binding protein type 2; mRNA cap-binding protein type 3

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Gene Aliases: 2700069E09Rik; 4E-LP; 4EHP; AI036339; AV129531; D0H0S6743E; EIF4E2; EIF4EL3; IF4e

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UniProt ID: (Human) O60573, (Mouse) Q8BMB3

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Entrez Gene ID: (Human) 9470, (Rat) 363275, (Mouse) 26987

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Function(s)
RNA cap binding translation initiation factor activity protein binding translation factor activity, RNA binding ubiquitin protein ligase binding poly(A) RNA binding RNA binding
Process(es)
in utero embryonic development translational initiation negative regulation of translation translation regulation of translation
It has to be done as per old AB suggested Products section.
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